Total phenolic, flavonoid contents, antioxidant and antimicrobial activities of Hesperis pendula

Falah Saleh Mohammed; Eylem Kına; İmran Uysal; Mustafa Sevindik

doi:10.56782/pps.135

Published : 2023-06-06

Vol. 21 No. 2 (2023)

Total phenolic, flavonoid contents, antioxidant and antimicrobial activities of Hesperis pendula

Falah Saleh Mohammed

Eylem Kına

İmran Uysal

Mustafa Sevindik

DOI: https://doi.org/10.56782/pps.135

Abstract

Many natural products are used in complementary medicine. Plants are widely used among these natural products. In this study, it was aimed to determine the total phenolic and flavonoid contents, total antioxidant status and antimicrobial activity of Hesperis pendula DC. In this context, the above-ground parts of the plant were extracted with ethanol and methanol. The total antioxidant level of the plant was determined using Rel Assay Diagnostics kits (Megatıp/Türkiye). The total phenolic content was assessed using the Folin-Ciocalteu reagent. Aluminum chloride assay was used to estimate the total flavonoid content. Antimicrobial activity was tested against bacterial and fungal strains by agar dilution method. As a result of the studies, it was observed that the ethanol extract of the plant had higher TAS (Total antioxidant status) (5.707±0.194 mmol/L), TOS (Total oxidant status) (21.646±0.239 µmol/L) and OSI (Oxidative stress index) (0.380±0.017) values. Total phenolic content was higher in ethanol extract (116.78±2.51 mg/g) while total flavonoid content was higher in methanol extract (93.64±2.16 mg/g). It was observed that the ethanol and methanol extracts of the plant inhibited the growth of bacteria at 100-200 µg/mL concentrations. It was determined that ethanol extract inhibited the growth of fungi at 200 µg/mL concentration and methanol extract at 200-400 µg/mL concentrations. In this context, it was determined that H. pendula could be a natural antioxidant and antimicrobial source.

Keywords:

Antimicrobial, Antioxidant, Hesperis, oxidant, total Phenolic, flavonoid

Similar Articles

Nazan Çömlekçioğlu, Nuh Korkmaz, Mehmet Ali Yüzbaşıoğlu, İmran Uysal, Mustafa Sevindik, Mistletoe (Loranthus europaeus Jacq.): antioxidant, antimicrobial and anticholinesterase activities , Prospects in Pharmaceutical Sciences: Vol. 22 No. 3 (2024)
Nuh Kormaz, Falah Saleh Mohammed, Imran Uysal, Mustafa Sevindik, Antioxidant, antimicrobial and anticholinesterase activity of Dittrichia graveolens , Prospects in Pharmaceutical Sciences: Vol. 21 No. 4 (2023)
Celal Bal, Emre Cem Eraslan, Mustafa Sevindik, Antioxidant, antimicrobial activities, total phenolic and element contents of wild edible mushroom Bovista nigrescens , Prospects in Pharmaceutical Sciences: Vol. 21 No. 2 (2023)
Nazan Comlekcioglu, Ashabil Aygan, Mustafa Sevindik, Uğur Comlekcioglu, Comparative analysis of phytochemical properties of extracts obtained from flowers and leaves of Astragalus angustifolius collected from different locations , Prospects in Pharmaceutical Sciences: Vol. 22 No. 3 (2024)
Nuh Korkmaz, Oğuzhan Koçer, Shahnaz Fathi, Imran Uysal, Mustafa Sevindik, Branched horsetail (Equisetum ramosissimum): some biological activities and total phenolic and flavonoid contents , Prospects in Pharmaceutical Sciences: Vol. 22 No. 3 (2024)
Ali İmran Korkmaz, Celal Bal, Emre Cem Eraslan, Mustafa Sevindik, Hasan Akgul, Biological activities of Agrocybe praecox (spring fieldcap mushroom) , Prospects in Pharmaceutical Sciences: Vol. 21 No. 4 (2023)
Arzu Birinci Yildirim, Ayca Cimen, Yavuz Baba, Arzu Turker, Natural- and in vitro-grown Filipendula ulmaria (L.) Maxim: evaluation of pharmaceutical potential (antibacterial, antioxidant and toxicity) and phenolic profiles , Prospects in Pharmaceutical Sciences: Vol. 22 No. 1 (2024)
Sükrü Canpolat, Cihan Düşgün, Elif Ildız, Elif Yürümez Canpolat, Cemil İşlek, Antioxidant potential and fatty acid composition of Lactarius sanguifluus , Prospects in Pharmaceutical Sciences: Vol. 22 No. 4 (2024)
Ali İmran Korkmaz, Celal Bal, Tetiana Krupodorova, Mehmet Ali Yüzbaşıoğlu, Beste Gizem Özbey Sarıdoğan, Mustafa Sevindik, Some biological activities and element levels of Lycoperdon pratense , Prospects in Pharmaceutical Sciences: Vol. 22 No. 2 (2024)
Ramona Figat, PHENOLIC ACIDS – ANTIGENOTOXIC COMPOUNDS FROM MEDICINAL AND EDIBLE PLANTS , Prospects in Pharmaceutical Sciences: Vol. 19 No. 4 (2021)

1 2 3 4 > >>

You may also start an advanced similarity search for this article.

Details

References

Statistics

Authors

Download files

pdf

Citation rules

Mohammed, F. S., Kına, E., Uysal, İmran, & Sevindik, M. (2023). Total phenolic, flavonoid contents, antioxidant and antimicrobial activities of Hesperis pendula. Prospects in Pharmaceutical Sciences, 21(2), 57–61. https://doi.org/10.56782/pps.135

Altmetric indicators

Cited by / Share

Licence

This work is licensed under a Creative Commons Attribution 4.0 International License.

References

Fitzgerald, M.; Heinrich, M.; Booker, A. Medicinal plant analysis: A historical and regional discussion of emergent complex techniques. Front. Pharmacol., 2020, 10, Art. No.: 1480. https://doi.org/10.3389/fphar.2019.01480 DOI: https://doi.org/10.3389/fphar.2019.01480

Foley, H.; Steel, A.; Cramer, H.; Wardle, J.; Adams, J. Disclosure of complementary medicine use to medical providers: a systematic review and meta-analysis. Sci. Rep., 2019, 9(1), 1573. https://doi.org/10.1038/s41598-018-38279-8 DOI: https://doi.org/10.1038/s41598-018-38279-8

Salmerón-Manzano, E., Garrido-Cardenas, J. A., Manzano-Agugliaro, F. (2020). Worldwide research trends on medicinal plants. Int. J. Environ. Res. Public Health., 2020, 17(10), 3376. https://doi.org/10.3390/ijerph17103376 DOI: https://doi.org/10.3390/ijerph17103376

Żero, P.; Niemyjska, M.; Rasztawicka, M.; Maciejewska, D. Breast cancer disease and new compounds with anticancer activity. Prospects Pharm. Sci., 2005, 3(2), 10-18. https://doi.org/10.56782/pps.53 DOI: https://doi.org/10.56782/pps.53

Kawka, M.; Pilarek, M.; Sykłowska-Baranek, K.; Pietrosiuk, A. In situ extraction of plant secondary metabolites. Prospects Pharm. Sci., 2017, 15(7), 60-67. https://doi.org/10.56782/pps.78 DOI: https://doi.org/10.56782/pps.78

Ghuman, S.; Ncube, B.; Finnie, J. F.; McGaw, L. J.; Njoya, E. M.; Coopoosamy, R. M.; Van Staden, J. Antioxidant, anti-inflammatory and wound healing properties of medicinal plant extracts used to treat wounds and dermatological disorders. S. Afr. J. Bot., 2019, 126, 232-240. https://doi.org/10.1016/j.sajb.2019.07.013 DOI: https://doi.org/10.1016/j.sajb.2019.07.013

Diorio, C.; Kelly, K. M.; Afungchwi, G. M.; Ladas, E. J.; Marjerrison, S. Nutritional traditional and complementary medicine strategies in pediatric cancer: A narrative review. Pediatr. Blood Cancer, 2020, 67, e28324. https://doi.org/10.1002/pbc.28324 DOI: https://doi.org/10.1002/pbc.28324

Korkmaz, N.; Dayangaç, A.; Sevindik, M. Antioxidant, antimicrobial and antiproliferative activities of Galium aparine. J. Fac. Pharm. Ankara, 2021, 45(3), 554-564. https://doi.org/10.33483/jfpau.977776 DOI: https://doi.org/10.33483/jfpau.977776

Lapava, N. Antiallergic Activity of Matricaria discoidea Herb (Asteraceae). Rastıtelnye Resursy Учредители: Российская академия наук, 2021, 57(4), 382-384. https://doi.org/10.31857/S0033994621040075 DOI: https://doi.org/10.31857/S0033994621040075

Mohammed, F. S.; Sevindik, M.; Uysal, I.; Sevindik, E.; Akgül, H. A Natural Material for Suppressing the Effects of Oxidative Stress: Biological Activities of Alcea kurdica. Biol. Bull., 2022, 49(2), S59-S66. http://dx.doi.org/10.1134/S1062359022140102 DOI: https://doi.org/10.1134/S1062359022140102

Uysal, I.; Koçer, O.; Mohammed, F. S.; Lekesiz, Ö.; Doğan, M.; Şabik, A. E.; Sevindik, E.; Gerçeker, F.Ö.; Sevindik, M. Pharmacological and Nutritional Properties: Genus Salvia. Adv. Pharmacol. Pharm., 2023, 11(2), 140-155 http://dx.doi.org/10.13189/app.2023.110206 DOI: https://doi.org/10.13189/app.2023.110206

Duran, A.; Martin, E.; Ünal, F. Chromosome numbers in the some taxa of Hesperis L. (Brassicaceae) from Turkey. Int. J. Nat. Sci., 2008, 2(1), 53-56.

Davis, P. H. Flora of Turkey and the East Aegean Islands, Volume 1. Edinburgh University Press, 1965.

Bauer, A.W.; Kirby, W.M.; Sherris, J.C.; Turck, M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 1966, 45, 493-96. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493

Hindler, J.; Hochstein, L.; Howell, A. Preparation of routine media and reagents used in antimicrobial susceptibility testing. Part 1. McFarland standards, p. 5.19.1-5.19.6. In H. D. Isenberg (ed) Clinical microbiology procedures handbook, vol. 1. American Society for Microbiology, Washington, D.C, 1992.

Matuschek, E.; Brown, D.F.; Kahlmeter, G. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin. Microbiol. Infect., 2014; 20, 255-266. https://doi.org/10.1111/1469-0691.12373 DOI: https://doi.org/10.1111/1469-0691.12373

Yumrutaş, Ö.; Saygideğer, S. D.; Doğan, M. The in vitro antioxidant activity of Allium tuncelianum: An endemic. J. Appl. Biol., 2009, 3(3), 61-64.

Chang, C. C.; Yang, M. H.; Wen, H. M.; Chern, J. C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 2002, 10(3), 178-182. DOI: https://doi.org/10.38212/2224-6614.2748

Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. biochem., 2004, 37(4), 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015 DOI: https://doi.org/10.1016/j.clinbiochem.2003.11.015

Erel, O. A new automated colorimetric method for measuring total oxidant status. Clin. biochem., 2005, 38(12), 1103-1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008 DOI: https://doi.org/10.1016/j.clinbiochem.2005.08.008

Sevindik, M. The novel biological tests on various extracts of Cerioporus varius. Fresenius Environ. Bull., 2019, 28(5), 3713-3717.

Sevindik, M. Antioxidant and antimicrobial capacity of Lactifluus rugatus and its antiproliferative activity on A549 cells. Indian J. Tradit. Knowl., 2020, 19(2), 423-427. DOI: https://doi.org/10.56042/ijtk.v19i2.35356

Islek, C.; Saridogan, B. G. O.; Sevindik, M.; Akata, I. Biological activities and heavy metal contents of some Pholiota species. Fresenius Environ. Bull., 2021, 30(6), 6109-6114.

Karaltı, İ.; Eraslan, E. C.; Sarıdoğan, B. G. Ö.; Akata, I.; Sevindik, M. Total Antioxidant, Antimicrobial, Antiproliferative Potentials and Element Contents of Wild Mushroom Candolleomyces candolleanus (Agaricomycetes) from Turkey. Int. J. Med. Mushrooms, 2022, 24(12), 69-76 https://doi.org/10.1615/intjmedmushrooms.2022045389 DOI: https://doi.org/10.1615/IntJMedMushrooms.2022045389

Şener, S. Ö.; Badem, M.; Kanbolat, Ş.; Korkmaz, N.; Özgen, U.; Aliyazıcıoğlu, R.; Karaoglu, Ş.A.; Terzioğlu, S. The Therapeutic Potency of Hesperis matronalis subsp. matronalis for Alzheimer's and Microbial Diseases. Süleyman Demirel Üniv. Sağ. Bil. Derg., 2021, 12(1), 38-43.

Borchardt, J. R.; Wyse, D. L.; Sheaffer, C. C.; Kauppi, K. L.; Fulcher, R. G.; Ehlke, N. J.; Biesboer, D.A.; Bey, R. F. Antimicrobial activity of native and naturalized plants of Minnesota and Wisconsin. J. Med. Plant Res., 2008, 2(5), 98-110.

Figat, R. Phenolic acids-antigenotoxic compounds from medicinal and edible plants. Prospects in Pharmaceutical Sciences, 2021, 19(4), 28-41. https://doi.org/10.56782/pps.9 DOI: https://doi.org/10.56782/pps.9

Ekşi, G.; Yılmaz, G.; Eruygur, N.; Ayaz, F. In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis ısatidea (Boiss.) DA German & Al-Shehbaz (Brassicaceae) and its Anatomy. Düzce Üniv. Bil. Teknol. Derg., 2021, 9(4), 1483-1492. https://doi.org/10.29130/dubited.931454 DOI: https://doi.org/10.29130/dubited.931454

Krupodorova, T.; Sevindik, M. Antioxidant potential and some mineral contents of wild edible mushroom Ramaria stricta. AgroLife Sci. J., 2020, 9(1), 186-191.

Baba, H.; Sevindik, M.; Dogan, M.; Akgül, H. Antioxidant, antimicrobial activities and heavy metal contents of some Myxomycetes. Fresenius Environ. Bull., 2020, 29(09), 7840-7846.

Bal, C.; Sevindik, M.; Akgul, H.; Selamoglu, Z. Oxidative stress index and antioxidant capacity of Lepista nuda collected from Gaziantep/Turkey. Sigma J. Engin. Nat. Sci., 2019, 37(1), 1-5. DOI: https://doi.org/10.1155/2020/5620484

Kostici, R.; Pisoschi, C. G.; Popescu, F.; Mogoşanu, G. D.; Biță, A.; Pîrvu, A. S.; Popescu, F. D. Antioxidant Action of Hesperis matronalis L. in Chronic Experimental Diabetes. Pharm. Chem. J., 2022, 1-15. https://doi.org/10.1007/s11094-022-02759-z DOI: https://doi.org/10.1007/s11094-022-02759-z

Sevindik, M.; Akgul, H.; Pehlivan, M.; Selamoglu, Z. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 2017, 26(7), 4757-4763.

Mohammed, F. S.; Akgul, H.; Sevindik, M.; Khaled, B. M. T. Phenolic content and biological activities of Rhus coriaria var. zebaria. Fresenius Environ. Bull., 2018, 27(8), 5694-5702.

Mohammed, F. S.; Karakaş, M.; Akgül, H.; Sevindik, M. Medicinal properties of Allium calocephalum collected from Gara Mountain (Iraq). Fresenius Environ. Bull., 2019, 28(10), 7419-7426.

Mohammed, F. S.; Kına, E.; Sevindik, M.; Doğan, M.; Pehlivan, M. Antioxidant and antimicrobial activities of ethanol extract of Helianthemum salicifolium (Cistaceae). Indian J. Nat. Prod. Resour., 2021, 12(3), 459-462.

Unal, O.; Eraslan, E. C.; Uysal, I.; Mohammed, F. S.; Sevindik, M.; Akgul, H. Biological activities and phenolic contents of Rumex scutatus collected from Turkey. Fresenius Environ. Bull., 2022, 31(7), 7341-7346.

Korkmaz, A. I.; Akgul, H.; Sevindik, M.; Selamoglu, Z. Study on determination of bioactive potentials of certain lichens. Acta Aliment., 2018, 47(1), 80-87. https://doi.org/10.1556/066.2018.47.1.10 DOI: https://doi.org/10.1556/066.2018.47.1.10

Falah Saleh Mohammed

falah.sindy@uoz.edu.krd

Affiliation:

Department of Biology, Faculty of Science, Zakho University, 42-001 Duhok, Iraq Iraq

Eylem Kına

eylem.kinaa@gmail.com

Affiliation:

Department of Biology, Faculty of Science and Literature, Gaziantep University, 27500 Gaziantep, Turkey Turkey

İmran Uysal

uysal-imran@hotmail.com

Affiliation:

Department of Food Processing, Bahçe Vocational School, Osmaniye Korkut Ata University, 80-000 Osmaniye, Türkiye Turkey

Mustafa Sevindik

sevindik27@gmail.com

Affiliation:

Department of Biology, Faculty of Science and Literature, Osmaniye Korkut Ata University, 80-000 Osmaniye, Türkiye Turkey

Total phenolic, flavonoid contents, antioxidant and antimicrobial activities of Hesperis pendula

Falah Saleh Mohammed

Eylem Kına

İmran Uysal

Mustafa Sevindik

Abstract

Keywords:

Most read articles by the same author(s)

Similar Articles

Falah Saleh Mohammed

Eylem Kına

İmran Uysal

Mustafa Sevindik