Lycium barbarum polysaccharide fraction – isolation from fruits and impact on the secretion of inflammatory mediators by human mononuclear cells and neutrophils
DOI:
https://doi.org/10.56782/pps.264Keywords:
cytokines, chemokines, immunostimulationAbstract
The barbary wolfberry fruit (Lycium barbarum L.) has been used in traditional Chinese medicine for centuries as an anti-aging, anti-fatigue, immune-supporting, and soothing agent for many age-related, mainly metabolic, oxidative, and inflammatory diseases. The purpose of the study was to demonstrate potential properties supporting the work of the immune system of the Lycium barbarum polysaccharides (LBP) fraction by studying their effect on the secretion of cytokines or chemokines by human peripheral blood mononuclear cells (PBMC) and neutrophils (PMN). The impact of LBP (5-100 µg/mL) on the secretion of cytokines or chemokines (IL-6, IL-10, TNF-α, IL-1β, IL-8) by PBMC and PMN was tested by enzyme-linked immunosorbent assay (ELISA). The potential cytotoxicity of LBP was determined by staining with propidium iodide (PI) with flow cytometry. The tested fraction did not show a significant influence on IL-10 and IL-8 secretion by PBMC and PMN, respectively. However, LBP at the highest concentration stimulated PBMC to increase the production of IL-6 and TNF-α. The highest efficiency of LBP was shown against the secretion of TNF-α and IL-1β by PMN.
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