CONCENTRATION OF MALONDIALDEHYDE IN BLOOD AS AN INDICATOR IN LIVER TRANSPLANTATION
DOI:
https://doi.org/10.56782/pps.113Keywords:
MDA, HPLC, oxidative stress, liver transplantation, cancer hepatocellular carcinomaAbstract
Liver transplants and tumors are associated with oxidative stress and its biological marker malondialdehyde (MDA), a product of lipid peroxidation. The aim of the study was to determine whether the level of MDA in the donor is useful as a prognostic marker of graft survival following transplantation. Using HPLC-UV, the concentration of free MDA was determined in blood serum of 20 transplant recipients suffering from hepatocellular carcinoma, and 20 donors prior to transplantation. Validation of the analytical method demonstrated its usefulness in the determination of free MDA concentration in blood serum. The measured MDA concentrations varied within either group, with a range of 0–48 µmol/L in recipients and 0–3.54 µmol/L in donors. Because of the low number of patients in both groups, the results are inconclusive with regard to possible differences between donors and recipients, even though the mean values were lower in donors than in recipients. Therefore, further studies are needed to decide whether the determination of MDA concentrations in donors constitutes a useful prognostic indicator of graft survival following liver transplantation.
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