Anti-cancer activity of phenyl-1,3,5-heptatriyne in human liver cancer
DOI:
https://doi.org/10.56782/pps.275Keywords:
liver cancer, phenyl-1,3,5-heptatriyne, apoptosis, anticancerAbstract
Bidens pilosa, commonly known as Ottrancedi, is a medicinal plant from the ancient period. It contains polyacetylenes, flavonoids, terpenoids, phenylpropanoids, which are well known molecules present in high concentration in medicinal plants with many antibacterial, antifungal and anticancer properties. To study the detailed mode of action of phenyl-1,3,5-heptatriyne which showed promising results on human liver cancer cell line HepG-2, we performed many assays like DNA fragmentation assay, Caspase-3 assay, Cell cycle analysis and fluorescent microscopic studies. During over studies we found that the molecule causes apoptosis in cancer cells lines by expressing high amount of Caspase-3 enzyme and it arrests the cells in G2/M phase. As it is already reported that the infusion of B. pilosa L. is non-toxic for single and repeated dose also for dermal structures so the molecule phenyl-1,3,5-heptatriyne can be a good candidate for a anticancer drug in future.
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1. Kumari, P.; Misra, K.; Sisodia, B.S.; Faridi, U.; Srivastava, S.; Luqman, S.; Darokar, M.P.; Negi, A.S.; Gupta, M.M.; Singh, S.C.; Kumar, J.K. A promising anticancer and antimalarial component from the leaves of Bidens pilosa. Planta Med. 2009, 75, 59-61. DOI: 10.1055/s-0028-1088362 DOI: https://doi.org/10.1055/s-0028-1088362
2. Chiang, Y.M.; Chang, C.L.T.; Chang, S.L.; Yang, W.C.; Shyur, L.F. Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator. J. Ethnopharmacol. 2007, 110, 532-538. DOI: 10.1016/j.jep.2006.10.007 DOI: https://doi.org/10.1016/j.jep.2006.10.007
3. Chang, S.L.; Chang, C.L.T.; Chiang, Y.M.; Hsieh, R.H.; Tzeng, C.R.; Wu, T.K.; Sytwu, H.K.; Shyur, L.F.; Yang, W.C. Polyacetylenic compounds and butanol fraction from Bidens pilosa can modulate the differentiation of helper T cells and prevent autoimmune diabetes in non-obese diabetic mice. Planta Med. 2004, 70, 1045-1051. DOI: 10.1055/s-2004-832645 DOI: https://doi.org/10.1055/s-2004-832645
4. Cai, J.; Shi, S.Y.; Cheng, F.; Wei, M.; Zou, K.; Yu, X.Q.; Chen, J.F. Polyacetylene isomers isolated from Bidens pilosa L. suppress the metastasis of gastric cancer cells by inhibiting Wnt/β-Catenin and Hippo/YAP signaling pathways. Molecules 2023, 28, Art. No: 1837. DOI: 10.3390/molecules28041837 DOI: https://doi.org/10.3390/molecules28041837
5. Cárdenas, M.B.; Álvarez, C.S.; Morgado, E.B.; Gutiérrez, M.G.; Monteagudo, G.L.; Suarez, O.S. Toxicological evaluation of an infusion of Bidens pilosa. Pharmacol. Online 2006, 3, 428-434.
6. Steenkamp, V.; Gouws, M.C. Cytotoxicity of six South African medicinal plant extracts used in the treatment of cancer. S. Afr. J. Bot. 2006, 72, 630-633. DOI: 10.1186/1472-6882-11-14 DOI: https://doi.org/10.1016/j.sajb.2006.02.004
7. Carmichael, J.; DeGraff, W.G.; Gazdar, A.F.; Minna, J.D.; Mitchell, J.B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987, 47, 936-942.
8. Herrmann, M.; Lorenz, H.M.; Voll, R.E.A.; Grünke, M.; Woith, W.; Kalden, J.R. A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res. 1994, 22, Art. No: 5506. DOI: 10.1093/nar/22.24.5506 DOI: https://doi.org/10.1093/nar/22.24.5506
9. Boquest, A.C.; Day, B.N.; Prather, R.S. Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells. Biol. Reprod. 1999, 60, 1013-1019. DOI: 10.1095/biolreprod60.4.1013 DOI: https://doi.org/10.1095/biolreprod60.4.1013
10. Lu, K.H.; Lue, K.H.; Liao, H.H.; Lin, K.L.; Chung, J.G. Induction of caspase-3-dependent apoptosis in human leukemia HL-60 cells by paclitaxel. Clin. Chim. Acta 2005, 357, 65-73. DOI: 10.1016/j.cccn.2005.02.003 DOI: https://doi.org/10.1016/j.cccn.2005.02.003
11. Jurisicova, A.; Varmuza, S.; Casper, R.F. Programmed cell death and human embryo fragmentation. MHR: Basic Sci. Reprod. Med. 1996, 2, 93-98. DOI: 10.1093/molehr/2.2.93 DOI: https://doi.org/10.1093/molehr/2.2.93
12. Kim, H.Y.; Akbar, M.; Lau, A.; Edsall, L. Inhibition of neuronal apoptosis by docosahexaenoic acid (22:6n-3): role of phosphatidylserine in antiapoptotic effect. J. Biol. Chem. 2000, 275, 35215-35223. DOI: 10.1074/jbc.M004446200 DOI: https://doi.org/10.1074/jbc.M004446200
13. Li, H.; Wang, L.J.; Qiu, G.F.; Yu, J.Q.; Liang, S.C.; Hu, X.M. Apoptosis of HeLa cells induced by extract from Cremanthodium humile. Food Chem. Toxicol. 2007, 45, 2040-2046. DOI: 10.1016/j.fct.2007.05.001 DOI: https://doi.org/10.1016/j.fct.2007.05.001
14. Weng, S.L.; Taylor, S.L.; Morshedi, M.; Schuffner, A.; Duran, E.H.; Beebe, S.; Oehninger, S. Caspase activity and apoptotic markers in ejaculated human sperm. Mol. Hum. Reprod. 2002, 8, 984-991. DOI: 10.1093/molehr/8.11.984 DOI: https://doi.org/10.1093/molehr/8.11.984
15. Arruebo, M.; Vilaboa, N.; Sáez-Gutierrez, B.; Lambea, J.; Tres, A.; Valladares, M.; González-Fernández, Á. Assessment of the evolution of cancer treatment therapies. Cancers 2011, 3, 3279-3330. DOI: 10.3390/cancers3033279 DOI: https://doi.org/10.3390/cancers3033279
16. Sakahira, H.; Enari, M.; Nagata, S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998, 391, 96-99. DOI: 10.1038/34214 DOI: https://doi.org/10.1038/34214
17. Janicke, R.U.; Sprengart, M.L.; Wati, M.R.; Porter, A.G. Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J. Biol. Chem. 1998, 273, 9357-9360. DOI: https://doi.org/10.1074/jbc.273.16.9357
18. Vermeulen, K.; Van Bockstaele, D.R.; Berneman, Z.N. The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif. 2003, 36, 131-149. DOI: 10.1046/j.1365-2184.2003.00266.x DOI: https://doi.org/10.1046/j.1365-2184.2003.00266.x
19. Shapiro, G.I.; Harper, J.W. Anticancer drug targets: cell cycle and checkpoint control. J. Clin. Invest. 1999, 104, 1645-1653, DOI: 10.1172/JCI9054 DOI: https://doi.org/10.1172/JCI9054
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