Comparative in vitro anti-inflammatory, wound-healing, enzyme-inhibitory, and antimicrobial activities of Boswellia dalzielii leaf and stem bark aqueous extracts

Authors

  • Seun Funmilola Akomolafe Ekiti State University and Medical University of Warsaw
  • Marcin Równicki Medical University of Warsaw
  • Weronika Skowrońska Medical University of Warsaw
  • Sebastian Granica Medical University of Warsaw

DOI:

https://doi.org/10.56782/pps.924

Keywords:

Boswellia dalzielii, keratinocytes, wound repair, skin inflammation, antimicrobial activity, matrix-degrading enzymes, traditional medicine

Abstract

Boswellia dalzielii (Burseraceae) (B. dalzielii) is traditionally used in West African communities to treat wounds, inflammatory skin disorders, mental disorder, and microbial infections, yet its dermatological potential has not been fully validated scientifically. This study evaluated the comparative in vitro cytocompatibility, wound-healing, anti-inflammatory, enzyme-inhibitory, and antibacterial activities of aqueous leaf (BDL) and stem bark (BDSB) extracts. Both extracts maintained high viability and normal morphology of HaCaT keratinocytes across tested concentrations (25–200 µg/mL) and enhanced cell migration in a scratch wound assay, with BDSB showing the greatest effect. Extracts significantly reduced TNF-α/IFN-γ-induced production of nitric oxide, IL-6 and IL-8 as well as inhibited collagenase, elastase, and hyaluronidase activities, indicating protection of extracellular matrix components. Antibacterial assessment revealed strong activity against Staphylococcus aureus (MIC = 0.31 mg/mL), whereas Pseudomonas aeruginosa was inhibited only at higher concentrations (10 mg/mL). Importantly, this represents the first comparative evaluation of B. dalzielii leaf and stem bark extracts across multiple skin-relevant in vitro activities, scientifically supporting its folkloric usage.

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Author Biographies

  • Seun Funmilola Akomolafe, Ekiti State University and Medical University of Warsaw

    Department of Biochemistry, Ekiti State University

    Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw

  • Marcin Równicki, Medical University of Warsaw

    Microbiota Lab, Department of Pharmaceutical Microbiology and Bioanalysis,

  • Weronika Skowrońska, Medical University of Warsaw

    Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

    Microbiota Lab, Department of Pharmaceutical Microbiology and Bioanalysis, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

     

  • Sebastian Granica, Medical University of Warsaw

    bDepartment of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

    cMicrobiota Lab, Department of Pharmaceutical Microbiology and Bioanalysis, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

     

References

1. Menon, G.K.; Kligman, A.M. Barrier functions of human skin: a holistic view. Skin Pharmacol. Physiol. 2009, 22(4), 178-189. DOI: 10.1159/000231523 DOI: https://doi.org/10.1159/000231523

2. Piipponen, M.; Li, D.; Landén, N.X. The immune functions of keratinocytes in skin wound healing. Int. J. Mol. Sci. 2020, 21(22), Art. No: 8790. DOI: 10.3390/ijms21228790 DOI: https://doi.org/10.3390/ijms21228790

3. Rashid, A.; Akram, M.; Kayode, O.T.; Kayode, A.A. Clinical features and epidemiological patterns of infections by multidrug resistance Staphylococcus aureus and Pseudomonas aeruginosa in patients with burns. Biomed. J. Sci. Tech. Res. 2020, 25(4), 19272-19278. DOI: 10.26717/BJSTR.2020.25.004222 DOI: https://doi.org/10.26717/BJSTR.2020.25.004222

4. Brunel, A.S.; Guery, B. Multidrug resistant (or antimicrobial-resistant) pathogens-alternatives to new antibiotics? Swiss Med. Wkly 2017, 147, Art. No: w14553. DOI: 10.4414/smw.2017.14553 DOI: https://doi.org/10.4414/smw.2017.14553

5. El-Saadony, M.T.; Saad, A.M.; Mohammed, D.M.; Korma, S.A.; Alshahrani, M.Y.; Ahmed, A.E.; Ibrahim, E.H.; Salem, H.M.; Alkafaas, S.S.; Saif, A.M.; Elkafas, S.S. Medicinal plants: bioactive compounds, biological activities, combating multidrug-resistant microorganisms, and human health benefits – a comprehensive review. Front. Immunol. 2025, 16, Art. No: 1491777. DOI: 10.3389/fimmu.2025.1491777 DOI: https://doi.org/10.3389/fimmu.2025.1491777

6. Rosique, R.G.; Rosique, M.J.; Farina Junior, J.A. Curbing inflammation in skin wound healing: a review. Int. J. Inflamm. 2015, 2015(1), Art. No: 316235. DOI: 10.1111/j.1742-4801.2006.00212.x DOI: https://doi.org/10.1155/2015/316235

7. Feliciani, C.; Gupta, A.K.; Saucier, D.N. Keratinocytes and cytokine/growth factors. Crit. Rev. Oral Biol. Med. 1996, 7(4), 300-318. DOI: 10.1177/‌10454411‌960070040101 DOI: https://doi.org/10.1177/10454411960070040101

8. Jiratchayamaethasakul, C.; Ding, Y.; Hwang, O.; Im, S.T.; Jang, Y.; Myung, S.W.; Lee, J.M.; Kim, H.S.; Ko, S.C.; Lee, S.H. In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fish. Aquat. Sci. 2020, 23(1), Art. No: 6. DOI: 0.1186/s41240-020-00149-8 DOI: https://doi.org/10.1186/s41240-020-00149-8

9. Tsioutsiou, E.E.; Amountzias, V.; Vontzalidou, A.; Dina, E.; Stevanović, Z.D.; Cheilari, A.; Aligiannis, N. Medicinal plants used traditionally for skin related problems in the south Balkan and east Mediterranean region—a review. Front. Pharmacol. 2022, 13, Art. No: 936047. DOI: 10.3389/fphar.2022.936047 DOI: https://doi.org/10.3389/fphar.2022.936047

10. Dogara, A.M.; Lema, A.A.; Hama, H.A.; Hamad, S.W.; Mahmod, N.H.; Khandaker, M.M.; Amlabu, W.E. Ethnopharmacology, biological evaluation and chemical composition of Boswellia dalzielii hutch: A review. Indones. J. Pharm. 2023, 33(4), 515 – 539. DOI: https://doi.org/10.22146/ijp.4279

11. Olukemi, M.A.; Kandakai-Olukemi, Y.T.; Mawak, J.D. Antibacterial activity of the stem bark of Boswellia dalzielii. J. Pharm. Bioresour. 2005, 2(2), 131-136. DOI: https://doi.org/10.4314/jpb.v2i2.32076

12. Otitoju, A.P.; Longdet, I.Y.; Alemika, T.E.; Adegoke, A.M.; Gota, V.P. Cytotoxic activity of Boswellia dalzielii (Hutch) stem bark extract against head and neck squamous cell carcinoma of the tongue (AW8507 cell line). J. Pharm. Bioresour. 2019, 16(2), 187-194. DOI: 10.1186/s43046-021-00075-3 DOI: https://doi.org/10.4314/jpb.v16i2.14

13. DeCarlo, A.; Dosoky, N.S.; Satyal, P.; Sorensen, A.; Setzer, W.N. The essential oils of the Burseraceae. In Essential Oil Research: Trends in Biosynthesis, Analytics, Industrial Applications and Biotechnological Production. 2019, pp. 61-145. Cham: Springer International Publishing. DOI: https://doi.org/10.1007/978-3-030-16546-8_4

14. Kubmarawa, D.; Ogunwande, I.A.; Okorie, D.A.; Olawore, N.O.; Kasali; A.A. Constituents of the essential oils of Boswellia dalzielii Hutch. Nigeria. J. Essent. Oil Res. 2006, 18, 119-120. DOI: 10.1080/‌10412905.2006.9699038 DOI: https://doi.org/10.1080/10412905.2006.9699038

15. Mamza, U.T.; Sodipo, O.A.; Abdulrahman, F.I.; Khan, I.Z. Phytochemical analysis and in vitro antimicrobial assay of the methanolic stem bark extract of Boswellia dalzielii Hutch (Burseraceae). J. Chem. Res. 2018, 3, 161-168.

16. Abdulhamid, A.; Sani, I. Phytochemical Screening and In-Vitro Antibacterial Potential of Boswellia dalzielii (Hutch.) Stem Bark Extracts. East African Scholars J. Med. Sci. 2019, 2, 213-217.

17. Abubakar, U.S.; Yusuf, K.M.; Abdu, G.T.; Saidu, S.R.; Jamila, G.A.; Fatima, A. Ethnopharmacological survey of medicinal plants used for the management of pediatric ailments in Kano State, Nigeria. Res. J. Pharmacogn. 2017, 4(3), 29-39.

18. Ohemu, T.L.; Agunu, A.; Olotu, P.N.; Ajima, U.; Dafam, D.G.; Azila, J.J. Ethnobotanical survey of medicinal plants used in the traditional treatment of viral infections in Jos, Plateau state, Nigeria. Int. J. Med. Arom. Plants. 2014, 4, 74-81.

19. Alemika, T.E.; Onawunmi, G.O.; Olugbade, T.A. Isolation and characterization of incensole from Boswellia dalzielii stem bark. J. Pharm. Bioresour. 2004, 1(1), 7-11. DOI: https://doi.org/10.4314/jpb.v1i1.32041

20. Kafuti, Y.S.; Ojerinde, S.O.; Balogun, O.; Alemika, T.E.; Taba, K.M.; Mpiana, P.T.; Kindombe, N.M. Antifungal evaluation and phytochemical screening of methanolic extract and fractions of Boswellia dalzielii stem bark. J. Pharm. Bioresour. 2017, 14(1), 62-67. DOI: https://doi.org/10.4314/jpb.v14i1.8

21. Mbiantcha, M.; Almas, J.; Atsamo, A.D.; Ateufack, G.; Shabana, S.U.; Bomba Tatsinkou, D.F.; Yousseu Nana, W.; Nida, D. Anti-inflammatory and anti-arthritic effects of methanol extract of the stem bark of Boswellia dalzielii hutch (Burseraceae) in rats. Inflammopharmacology 2018, 26(6), 1383-1398. DOI: 10.1007/s10787-018-0505-x DOI: https://doi.org/10.1007/s10787-018-0505-x

22. Nazifi, A.B.; Danjuma, N.M.; Olurishe, T.O.; Ya’u, J. Behavioural effects of methanol stem bark extract of Boswellia dalzielii Hutch (Burseraceae) in mice. Afr. J. Biomed. Res. 2017, 20(1), 103-108. DOI: https://doi.org/10.66563/7vs9m342

23. Salihu, T.; Olukunle, J.O.; Adenubi, O.T.; Mbaoji, C.; Zarma, M.H. Ethnomedicinal plant species commonly used to manage arthritis in North-West Nigeria. S. Afr. J. Bot. 2018, 118, 33-43. DOI: 10.1016/j.sajb.‌2018.06.004 DOI: https://doi.org/10.1016/j.sajb.2018.06.004

24. Akomolafe, S. F.; Fabjanowicz, M.; Granica; S. Comprehensive analysis of antioxidant activity and phytochemical profile of leaf, stem bark, and root bark aqueous extracts of Boswellia dalzielii Hutch: an in vitro and UHPLC-DAD-MS studies. Acta Soc. Bot. Pol. 2025, 94, Art. No: 212105. DOI: 10.5586/asbp/212105 DOI: https://doi.org/10.5586/asbp/212105

25. Ohemu, T.; Okwori, V.; Dafam, D.; Kagaru, D.; Yakubu, T.; Usman, Y. Pharmacognostic and proximate evaluations of the leaves, stem bark and roots of Boswellia dalzielii Hutch (Burseraceae). Nig. J. Prod. Med. 2018, 22, 115-123. DOI: 10.4314/njnpm.v22i1.4

26. Ndlovu, G.; Fouche, G.; Tselanyane, M.; Cordier, W.; Steenkamp, V. In vitro determination of the anti-aging potential of four southern African medicinal plants. BMC Complement. Altern. Med. 2013, 13(1), Art. No: 304. DOI: 10.1111/j.1365-4362.1996.tb03650.x DOI: https://doi.org/10.1186/1472-6882-13-304

27. Jakupović, L.; Bačić, I.; Jablan, J.; Marguí, E.; Marijan, M.; Inić, S.; Nižić Nodilo, L.; Hafner, A.; Zovko Končić, M. Hydroxypropyl-β-Cyclodextrin-Based Helichrysum italicum extracts: antioxidant and cosmeceutical activity and biocompatibility. Antioxidants 2023, 12, Art. No: 855. DOI: 10.3390/‌antiox12040855 DOI: https://doi.org/10.3390/antiox12040855

28. Van Wart, H.E.; Steinbrink, D.R. A continuous spectrophotometric assay for Clostridium histolyticum collagenase. Anal. Biochem. 1981, 113(2), 356-365. DOI: 10.1016/0003-2697(81)90089-0 DOI: https://doi.org/10.1016/0003-2697(81)90089-0

29. Ghuman, S.; Ncube, B.; Finnie, J.F.; McGaw, L.J.; Coopoosamy, R.M.; Van Staden, J. Antimicrobial activity, phenolic content, and cytotoxicity of medicinal plant extracts used for treating dermatological diseases and wound healing in KwaZulu-Natal, South Africa. Front. Pharmacol. 2016, 7, Art. No: 320. DOI: 10.3389/fphar.2016.00320 DOI: https://doi.org/10.3389/fphar.2016.00320

30. Kohoude, M.J.; Gbaguidi, F.; Agbani, P.; Ayedoun, M.A.; Cazaux, S.; Bouajila, J. Chemical composition and biological activities of extracts and essential oil of Boswellia dalzielii leaves. Pharm. Biol. 2017, 55(1), 33-42. DOI: 10.1080/13880209.2016.1226356 DOI: https://doi.org/10.1080/13880209.2016.1226356

31. Ahmed, H.H.; Abd-Rabou, A.A.; Hassan, A.Z.; Kotob, S.E. Phytochemical analysis and anti-cancer investigation of Boswellia serrata bioactive constituents in vitro. Asian Pac. J. Cancer Prev. 2015, 16(16), 7179-7188. DOI: 10.7314/apjcp.2015.‌16.16.7179 DOI: https://doi.org/10.7314/APJCP.2015.16.16.7179

32. Majeed, M.; Nagabhushanam, K.; Lawrence, L.; Nallathambi, R.; Thiyagarajan, V.; Mundkur, L. Boswellia serrata extract containing 30% 3-acetyl-11-keto-boswellic acid attenuates inflammatory mediators and preserves extracellular matrix in collagen-induced arthritis. Front. Physiol. 2021, 12, Art. No: 735247. DOI: 10.3389/fphys.2021.735247 DOI: https://doi.org/10.3389/fphys.2021.735247

33. Bártolo, I.; Reis, R.L.; Marques, A.P.; Cerqueira, M.T. Keratinocyte growth factor-based strategies for wound re-epithelialization. Tissue Eng. Part B Rev. 2022, 28(3), 665-676. DOI: 10.1089/ten.teb.2021.0030 DOI: https://doi.org/10.1089/ten.teb.2021.0030

34. Schilrreff, P.; Alexiev, U. Chronic inflammation in non-healing skin wounds and promising natural bioactive compounds treatment. Int. J. Mol. Sci. 2022, 23(9), Art. No: 4928. DOI: 10.3390/ijms23094928 DOI: https://doi.org/10.3390/ijms23094928

35. Alqarni, A. Evaluation of the antibacterial, anti-inflammatory and wound healing properties of frankincense (Boswellia frereana) 2025, (Doctoral dissertation, Cardiff University).

36. Shedoeva, A.; Leavesley, D.; Upton, Z.; Fan, C. Wound healing and the use of medicinal plants. eCAM 2019, 2019(1), Art. No: 2684108. DOI: 10.1155/‌2019/2684108 DOI: https://doi.org/10.1155/2019/2684108

37. Anzar, C.A.; Joseph, M.V.; Sundaram, R.; Vadiraj, G.B.; Prasad, C.P.; Eranimose, B. A novel therapeutic effect of the wound healing activity of Boswellia serrata extract (boswegex®) by using the human dermal fibroblast (HDF) cell line: an in-vitro study. Int. J. Pharm. Sci. Res. 2024, 15, 756-762. DOI: 10.13040/IJPSR.0975-8232.15(3).756-62 DOI: https://doi.org/10.13040/IJPSR.0975-8232.15(3).756-62

38. Jiang, Y.; Tsoi, L.C.; Billi, A.C.; Ward, N.L.; Harms, P.W.; Zeng, C.; Maverakis, E.; Kahlenberg, J.M.; Gudjonsson, J.E. Cytokinocytes: the diverse contribution of keratinocytes to immune responses in skin. JCI Insight 2020, 5(20), Art. No: e142067. DOI: 10.1172/jci.insight.142067 DOI: https://doi.org/10.1172/jci.insight.142067

39. Ammon, H.P.T. Boswellic acids and their role in chronic inflammatory diseases. Adv. Exp. Med. Biol. 2016, 928, 291-327. DOI: 10.1007/978-3-319-41334-1_13 DOI: https://doi.org/10.1007/978-3-319-41334-1_13

40. T sai, Y.C.; Chang, H.H.; Chou, S.C.; Chu, T.W.; Hsu, Y.J.; Hsiao, C.Y.; Lo, Y.H.; Wu, N.L.; Chang, D.C.; Hung, C.F. Evaluation of the anti-atopic dermatitis effects of α-boswellic acid on Tnf-α/Ifn-γ-Stimulated HaCat Cells and DNCB-Induced BALB/c Mice. Int. J. Mol. Sci. 2022, 23(17), Art. No: 9863. DOI: 10.3390/‌ijms23179863 DOI: https://doi.org/10.3390/ijms23179863

41. Hamadjida, A.; Mbomo, R.E.A.; Minko, S.E.; Ntchapda, F.; Mingoas, J.P.K.; Nnanga, N. Antioxidant and anti-inflammatory effects of Boswellia dalzielii and Hibiscus sabdariffa extracts in alloxan-induced diabetic rats. Metabol. Open 2024, 21, Art. No: 100278. DOI: 10.1016/j.metop.2024.100278 DOI: https://doi.org/10.1016/j.metop.2024.100278

42. Medzhitov, R. Origin and physiological roles of inflammation. Nature 2008, 454(7203), 428-435. DOI: 10.1038/nature07201 DOI: https://doi.org/10.1038/nature07201

43. Xie, Q.W.; Nathan, C. The high-output nitric oxide pathway: role and regulation. J. Leukoc. Biol. 1994, 56(5), 576-582. DOI: 10.1002/jlb.56.5.576 DOI: https://doi.org/10.1002/jlb.56.5.576

44. Bogdan, C. Nitric oxide and the immune response. Nat. Immunol. 2001, 2(10), 907-916. DOI: 10.1038/ni1001-907 DOI: https://doi.org/10.1038/ni1001-907

45. Kannan, G.; Paul, B.M.; Thangaraj, P. Stimulation, regulation, and inflammaging interventions of natural compounds on nuclear factor kappa B (NF-kB) pathway: a comprehensive review. Inflammopharmacology 2025, 33(1), 145-162. DOI: 10.1007/s10787-024-01635-4 DOI: https://doi.org/10.1007/s10787-024-01635-4

46. Al-Khayri, J.M.; Mascarenhas, R.; Harish, H.M.; Gowda, Y.; Lakshmaiah, V.V.; Nagella, P.; Al-Mssallem, M.Q.; Alessa, F.M.; Almaghasla, M.I.; Rezk, A.A.S. Stilbenes, a versatile class of natural metabolites for inflammation—an overview. Molecules 2023, 28(9), Art. No: 3786. DOI: 10.3390/‌molecules28093786 DOI: https://doi.org/10.3390/molecules28093786

47. Koh, Y.C.; Ho, C.T.; Pan, M.H. Recent advances in health benefits of stilbenoids. J. Agric. Food Chem. 2021, 69(35), 10036-10057. DOI: 10.1021/acs.jafc.‌1c03699 DOI: https://doi.org/10.1021/acs.jafc.1c03699

48. Ammon, H.P.T. Modulation of the immune system by Boswellia serrata extracts and boswellic acids. Phytomedicine 2010, 17(11), 862-867. DOI: 10.1016/‌j.phymed.2010.03.003 DOI: https://doi.org/10.1016/j.phymed.2010.03.003

49. Sharma, A.; Bhatia, S.; Kharya, M.D.; Gajbhiye, V.; Ganesh, N.; Namdeo, A.G.; Mahadik, K.R. Anti-inflammatory and analgesic activity of different fractions of Boswellia serrata. Int. J. Phytomed. 2010, 2(1), 94-99. DOI: 10.5138/ijpm.2010.0975.0185.02015

50. Safayhi, H.; Rall, B.; Sailer, E.R.; Ammon, H.P. Inhibition by boswellic acids of human leukocyte elastase. J. Pharmacol. Exp. Ther. 1997, 281(1), 460-463. DOI: 10.1016/S0022-3565(24)36628-5 DOI: https://doi.org/10.1016/S0022-3565(24)36628-5

51. Siemoneit, U.; Koeberle, A.; Rossi, A.; Dehm, F.; Verhoff, M.; Reckel, S.; Maier, T.J.; Jauch, J.; Northoff, H.; Bernhard, F.; Doetsch, V. Inhibition of microsomal prostaglandin E2 synthase‐1 as a molecular basis for the anti‐inflammatory actions of boswellic acids from frankincense. Br. J. Pharmacol. 2011, 162(1), 147-162. DOI: 10.1111/j.1476-5381.2010.01020.x DOI: https://doi.org/10.1111/j.1476-5381.2010.01020.x

52. Ammon, H.P.T. Boswellic acids in chronic inflammatory diseases. Planta Med. 2006, 72(12), 1100-1116. DOI: 10.1055/s-2006-947227 DOI: https://doi.org/10.1055/s-2006-947227

53. Efron, D.T.; Most, D.; Barbul, A. Role of nitric oxide in wound healing. Curr. Opin. Clin. Nutr. Metab. Care. 2000, 3(3), 197-204. DOI: 10.1177/1099800‌402004001002 DOI: https://doi.org/10.1097/00075197-200005000-00006

54. Potekaev, N.N.; Borzykh, O.B.; Medvedev, G.V.; Pushkin, D.V.; Petrova, M.M.; Petrov, A.V.; Dmitrenko, D.V.; Karpova, E.I.; Demina, O.M.; Shnayder, N.A. The role of extracellular matrix in skin wound healing. J. Clin. Med. 2021, 10(24), Art. No: 5947. DOI: 10.1007/s00403-009-1014-y DOI: https://doi.org/10.3390/jcm10245947

55. Ashruf, O.S.; Ansari, M.Y. Natural compounds: potential therapeutics for the inhibition of cartilage matrix degradation in osteoarthritis. Life 2022, 13(1), Art. No: 102. DOI: 10.3390/life13010102 DOI: https://doi.org/10.3390/life13010102

56. Biswas, P.C.; Saroj, B.K.; Raju, S.K.; Konda,VVS K.; Mukherjee, R.K.; P, N.; Jilani, A.K.; Jilani, A.K.; Kx, S. Antioxidant And Anti-Inflammatory Activities Of Boswellia Serrata Leaf Extract: A Pharmacological Perspective. Rev. Electron. Vet. 2024, 25(1), 1451 -1460. DOI: 10.69980/redvet.v25i1.925

57. Ratnam, K.V.; Bhakshu, L.M. Traditional uses and pharmacology of Boswellia species. In Frankincense–Gum Olibanum 2023, pp. 151-214. Apple Academic Press. DOI: 10.1201/9781003348016-7 DOI: https://doi.org/10.1201/9781003348016-7

58. Umar, S.; Umar, K.; Sarwar, A.H.M.G.; Khan, A.; Ahmad, N.; Ahmad, S.; Katiyar, C.K.; Husain, S.A.; Khan, H.A. Boswellia serrata extract attenuates inflammatory mediators and oxidative stress in collagen induced arthritis. Phytomedicine 2014, 21(6), 847-856. DOI: 10.1016/j.phymed.2014.03.001 DOI: https://doi.org/10.1016/j.phymed.2014.02.001

59. Lee, K.K.; Cho, J.J.; Park, E.J.; Choi, J.D. Anti‐elastase and anti‐hyaluronidase of phenolic substance from Areca catechu as a new anti‐ageing agent. Int. J. Cosmet. Sci. 2001, 23(6), 341-346. DOI: 10.1046/j.0412-5463.2001.00102.x DOI: https://doi.org/10.1046/j.0412-5463.2001.00102.x

60. Vestweber, P.K.; Wächter, J.; Planz, V.; Jung, N.; Windbergs, M. The interplay of Pseudomonas aeruginosa and Staphylococcus aureus in dual-species biofilms impacts development, antibiotic resistance and virulence of biofilms in in vitro wound infection models. PLOS One 2024, 19(5), Art. No: e0304491. DOI: 10.1371/journal.pone.0304491 DOI: https://doi.org/10.1371/journal.pone.0304491

61. Todorović, P.; Krstić Ristivojević, M.; Jović, M.; Ivković, Đ.; Nestorović Živković, J.; Gašić, U.; Dimkić, I.; Stojiljković, I.; Ristivojević, P. Antimicrobial Effect of Boswellia serrata Resin’s Methanolic Extracts Against Skin Infection Pathogens. Processes 2025, 13(3), Art. No: 850. DOI: 10.3390/pr13030850 DOI: https://doi.org/10.3390/pr13030850

62. Hasson, S.S.; Al-Balushi, M.S.; Sallam, T.A.; Idris, M.A.; Habbal, O.; Al-Jabri, A.A. In vitro antibacterial activity of three medicinal plants-Boswellia (Luban) species. Asian Pac. J. Trop. Biomed. 2011, 1(2), S178-S182. DOI: 10.13005/bbra/2570 DOI: https://doi.org/10.1016/S2221-1691(11)60151-2

63. Salisu, B.U.; Muhammad, A.M.; Mohammed, A.H.; Adamu, U. Determination of phytochemicals and antimicrobial activity of aqueous stem bark extract of Boswellia dalzielii against some common pathogenic microorganisms. UJMR 2017, 2(1), 238-246. DOI: https://doi.org/10.47430/ujmr.1721.035

64. El-sayed, N.R.; Samir, R.; Jamil M. Abdel-Hafez, L.; Ramadan, M.A. Olive leaf extract modulates quorum sensing genes and biofilm formation in multi-drug resistant Pseudomonas aeruginosa. Antibiotics 2020, 9(9), Art. No: 526. DOI: 10.3390/antibiotics9090526 DOI: https://doi.org/10.3390/antibiotics9090526

65. Piegerová, A.; Koščová, J.; Schusterová, P.; Nemcová, R.; Kryvtsova, M. In vitro inhibition of biofilm formation by Staphylococcus aureus under the action of selected plant extracts. Folia Vet. 2019, 63, 48–53. DOI: 10.2478/fv-2019-0007 DOI: https://doi.org/10.2478/fv-2019-0007

66. Tegasne, C.; Kapche, G.D.W.F.; Mawabo, I.K.; Talla, R.M.; Jouda, J.B.; Happi, G.M.; Lenta, B.N.; Frese, M.; Abegaz, B.M.; Sewald, N. Bioguided chemical study of Boswellia dalzielii Hutch. (Burseraceae) for antibacterial agents and a new glucopyranoxylmethoxybenzyle. Nat. Prod. Res. 2021, 35(23), 5199-520. DOI: 10.1080/‌14786419.‌2020.1794863 DOI: https://doi.org/10.1080/14786419.2020.1794863

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2026-09-05

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How to Cite

Comparative in vitro anti-inflammatory, wound-healing, enzyme-inhibitory, and antimicrobial activities of Boswellia dalzielii leaf and stem bark aqueous extracts. (2026). Prospects in Pharmaceutical Sciences, 24(3), 28-44. https://doi.org/10.56782/pps.924
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