The effect of ethyl acetate fraction of Vernonia amygdalina Del. leaves on uric acid levels of male white mice (Mus musculus) hyperuricemia

Rafifah Azzahra; Helmi Arifin; Dian Ayu Juwita

doi:10.56782/pps.259

Published : 2024-12-14

Vol. 22 No. 4 (2024)

The effect of ethyl acetate fraction of Vernonia amygdalina Del. leaves on uric acid levels of male white mice (Mus musculus) hyperuricemia

Rafifah Azzahra

Helmi Arifin

Dian Ayu Juwita

DOI: https://doi.org/10.56782/pps.259

Abstract

Hyperuricemia is a condition where uric acid levels in the blood are increased above normal. High levels of uric acid in the blood can cause a buildup of uric acid crystals in the joints and other organs of the body. One of the plants used as traditional medicine to reduce uric acid levels in the blood is Vernonia amygdalina Del. plant. This study aims to determine the effect of ethyl acetate fraction of V. amygdalina on uric acid levels of hyperuricemic male white mice. The experimental animals used were 54 male white mice which were divided into 6 groups, namely the negative control group, the positive control group, the comparison group, the 12.5 mg/kgBW dose treatment group, the 25 mg/kgBW treatment group, and the 50 mg/kgBW doses treatment group with a treatment duration of 5 days, 10 days and 15 days. Data analysis used two-way analysis of variance (ANOVA) followed by Duncan's Post Hoc test with 95% confidence intervals (P<0.05). The results obtained showed that uric acid levels decreased along with the length of time the ethyl acetate fraction was administered with the optimal reduction effect at a dose of 50 mg/kgBW. This study shows that the ethyl acetate fraction of V. amygdalina exhibits antihyperuricemic activity and can be used as an alternative medicine in the community.

Keywords:

Ethyl acetate fraction, Vernonia amygdalina Del. leaves, Uric Acid, Hyperuricemia

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Azzahra, R., Arifin, H., & Juwita, D. A. (2024). The effect of ethyl acetate fraction of Vernonia amygdalina Del. leaves on uric acid levels of male white mice (Mus musculus) hyperuricemia . Prospects in Pharmaceutical Sciences, 22(4), 99–104. https://doi.org/10.56782/pps.259

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Rafifah Azzahra

rafifahazzahra48@gmail.com

Helmi Arifin

Affiliation:

Faculty of Pharmacy, Andalas University, 25163 Padang, Indonesia Indonesia

Dian Ayu Juwita

Affiliation:

Faculty of Pharmacy, Andalas University, 25163 Padang, Indonesia Indonesia