IN SITU EXTRACTION OF PLANT SECONDARY METABOLITES
DOI:
https://doi.org/10.56782/pps.78Keywords:
in situ extraction, two-phase cultures, liquid perfluorochemicalsAbstract
Besides being a method to isolate plant metabolites from an in vitro culture, in situ extraction may enhance the biosynthesis of such metabolites in cultured plant biomass. In keeping with its definition, the process is based on the extraction of secondary metabolites, as they are being formed, via their accumulation in an additional phase of the culture system. Driven by the potential benefits attributable to this process, the search for in situ extractants and adsorbents that are non-toxic to the biomass and selective for the secondary metabolites yielded numerous candidate materials described in the scientific literature. A relatively new approach is the use of liquid perfluorochemicals (PFC) as the additional in situ extraction phase. Previously known as efficient carriers of gases, PFCs do not interact in any significant way with molecules dissolved in them. This results in a facile release of gases across the liquid-liquid interface of the culture system. Moreover, being immiscible with water, PFCs form in the presence of an aqueous culture medium a separate, lower phase. These properties of PFCs, in combination with a lack of toxicity toward living cells and a selectivity for plant metabolites, explain the mounting interest in the application of liquid perfluorochemicals as in situ extractants.
Downloads
References
Kawka M., Ekstrakcja in situ naftochinonów w hodowli korzeni Rindera graeca (In situ extraction of naphthoquinones in Rindera graeca transformed hairy root cultures). Praca dyplomowa na stopień magistra inżyniera wykonana w Zakładzie Biotechnologii i Inżynierii Bioprocesowej Wydziału Inżynierii Chemicznej i Procesowej Politechniki Warszawskiej i w Zakładzie Biologii Farmaceutycznej i Biotechnologii Roślin Leczniczych Wydziału Farmaceutycznego z Oddziałem Medycyny Laboratoryjnej Warszawskiego Uniwersytetu Medycznego 2016.
Malik S., Hossein Mirjalili M., Fett-Neto A. G., Mazzafera P. & Bonfill M., Living between two worlds: Two-phase culture systems for producing plant secondary metabolites, Critical Reviews in Biotechnology, 2013, 33, 1–22. DOI: https://doi.org/10.3109/07388551.2012.659173
Murthy H. N., Lee E.-J. & Paek K.-Y., Production of secondary metabolites from cell and organ cultures: Strategies and approaches for biomass improvement and metabolite accumulation, Plant Cell, Tissue and Organ Culture, 2014, 118, 1–16. DOI: https://doi.org/10.1007/s11240-014-0467-7
Pilarek M., Liquid perfluorochemicals as flexible and efficient gas carriers applied in bioprocess engineering: An updated overview and future prospects, Chemical and Process Engineering - Inzynieria Chemiczna i Procesowa, 2014, 35, 463–487. DOI: https://doi.org/10.2478/cpe-2014-0035
Sykłowska-Baranek K., Pilarek M., Cichosz M. & Pietrosiuk A., Liquid perfluorodecalin application for in situ extraction and enhanced naphthoquinones production in Arnebia euchroma cell suspension cultures, Applied Biochemistry and Biotechnology, 2014, 172, 2618–2627. DOI: https://doi.org/10.1007/s12010-013-0701-5
Azmir J., Zaidul I. S. M., Rahman M. M., Sharif K. M., Mohamed A., Jahurul M. H. A., Ghafoor K., Norulaini N. A. N. & Omar A. K. M., Techniques for extraction of bioactive compounds from plant materials - A review, Journal of Food Engineering, 2013, 117, 426–436. DOI: https://doi.org/10.1016/j.jfoodeng.2013.01.014
Ciborowski J., Inżynieria procesowa, Warszawa : Wydawnictwa Naukowo-Techniczne, 1973.
Kukuła-Koch W., Koch W. & Głowniak K., Novel extraction techniques towards the recovery of plant derived secondary metabolites - A review, Annales Universitatis Mariae Curie-Sklodowska, Sectio DDD: Pharmacia, 2011, 24, 83–94.
Incorvia Mattina M. J., Iannucci Berger W. A. & Denson C. L., Microwave-assisted extraction of taxanes from Taxus biomass, Journal of Agricultural and Food Chemistry, 1997, 45, 4691–4696. DOI: https://doi.org/10.1021/jf970454o
Verma A., Hartonen K. & Riekkola M.-L., Optimisation of supercritical fluid extraction of indole alkaloids from Catharanthus roseus using experimental design methodology - Comparison with other extraction techniques, Phytochemical Analysis, 2008, 19, 52–63. DOI: https://doi.org/10.1002/pca.1015
Ruffoni B., Pistelli L., Bertoli A. & Pistelli L., Plant cell cultures: Bioreactors for industrial production. W: Giardi M. T., Rea G., Berra B., Bio-Farms for nutraceuticals (s. 203-221), Advances in experimental medicine and biology 2010. DOI: https://doi.org/10.1007/978-1-4419-7347-4_15
Bertoli A., Giovannini A., Ruffoni B., Di Guardo A., Spinelli G., Mazzetti M. & Pistelli L., Bioactive constituent production in St. John’s Wort in vitro hairy roots. Regenerated plant lines, Journal of Agricultural and Food Chemistry, 2008, 56, 5078–5082. DOI: https://doi.org/10.1021/jf0729107
Grajek W., Biosynteza metabolitów wtórnych w kulturach in vitro. W: Malepszy S. (red.), Biotechnologia roślin (s. 306-340), Wydawnictwo Naukowe PWN 2007.
Ramachandra Rao S. & Ravishankar G. A., Plant cell cultures: Chemical factories of secondary metabolites, Biotechnology Advances, 2002, 20, 101–153. DOI: https://doi.org/10.1016/S0734-9750(02)00007-1
Robins R. J. & Rhodes M. J. C., The stimulation of anthraquinone production by Cinchona ledgeriana cultures with polymeric adsorbents, Applied Microbiology and Biotechnology, 1986, 24, 35–41. DOI: https://doi.org/10.1007/BF00266282
Kwon I. C., Yoo Y. J., Lee J. H. & Hyun J. O., Enhancement of taxol production by in situ recovery of product, Process Biochemistry, 1998, 33, 701–707. DOI: https://doi.org/10.1016/S0032-9592(98)00037-5
Hussain M. S., Fareed S., Ansari S., Rahman M. A., Ahmad I. Z. & Saeed M., Current approaches toward production of secondary plant metabolites, Journal of Pharmacy and Bioallied Sciences, 2012, 4, 10–20. DOI: https://doi.org/10.4103/0975-7406.92725
Kajani A. A., Mofid M. R., Abolfazli K. & Tafreshi S. A. H., Encapsulated activated charcoal as a potent agent for improving taxane synthesis and recovery from cultures, Biotechnology and Applied Biochemistry, 2010, 56, 71–76. DOI: https://doi.org/10.1042/BA20090344
Berlin J., Witte L., Schubert W. & Wray V., Determination and quantification of monoterpenoids secreted into the medium of cell cultures of Thuja occidentalis, Phytochemistry, 1984, 23, 1277–1279. DOI: https://doi.org/10.1016/S0031-9422(00)80441-0
Dörnenburg H. & Knorr D., Semicontinuous processes for anthraquinone production with immobilized Cruciata glabra cell cultures in a three-phase system, Journal of Biotechnology, 1996, 50, 55–62. DOI: https://doi.org/10.1016/0168-1656(96)01549-0
Kim D. J. & Chang H. N., Enhanced shikonin production from Lithospermum erythrorhizon by in situ extraction and calcium alginate immobilization, Biotechnology and Bioengineering, 1990, 36, 460–466. DOI: https://doi.org/10.1002/bit.260360505
Xuqing F. & Dewei L., Enhancement of shikonin production in cell suspension cultures of Arnebia euchroma employing two-liquid-phase systems, Chinese Journal of Chemical Engineering, 1998, 6, 86–90.
Yuan Y.-J., Wei Z.-J., Wu Z.-L. & Wu J.-C., Improved Taxol production in suspension cultures of Taxus chinensis var. mairei by in situ extraction combined with precursor feeding and additional carbon source introduction in an airlift loop reactor, Biotechnology Letters, 2001, 23, 1659–1662.
Tikhomiroff C., Allais S., Klvana M., Hisiger S. & Jolicoeur M., Continuous selective extraction of secondary metabolites from Catharanthus roseus hairy roots with silicon oil in a two-liquid-phase bioreactor, Biotechnology Progress, 2002, 18, 1003–1009. DOI: https://doi.org/10.1021/bp0255558
Bae G. W., Chung C. S., Kim K. I., Park C. H., Lee H. J., Chae Y. A. & Chung I. S., Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane, Biotechnology Techniques, 1998, 12, 843–845. DOI: https://doi.org/10.1023/A:1008800331295
Lin L. & Wu J., Enhancement of shikonin production in single- and two-phase suspension cultures of Lithospermum erythrorhizon cells using low-energy ultrasound, Biotechnology and Bioengineering, 2002, 78, 81–88. DOI: https://doi.org/10.1002/bit.10180
Deno H., Suga C., Morimoto T. & Fujita Y., Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon - VI. Production of shikonin derivatives by a two-layer culture containing an organic solvent, Plant Cell Reports, 1987, 6, 197–199. DOI: https://doi.org/10.1007/BF00268478
Zare K., Nazemiyeh H., Movafeghi A., Khosrowshahli M., Motallebi-Azar A., Dadpour M. & Omidi Y., Bioprocess engineering of Echium italicum L.: Induction of shikonin and alkannin derivatives by two-liquid-phase suspension cultures, Plant Cell, Tissue and Organ Culture, 2010, 100, 157–164. DOI: https://doi.org/10.1007/s11240-009-9631-x
Kumar R., Sharma N., Malik S., Bhushan S., Sharma U. K., Kumari D., Sinha A. K., Sharma M. & Ahuja P. S., Cell suspension culture of Arnebia euchroma (Royle) Johnston - a potential source of naphthoquinone pigments, Journal of Medicinal Plant Research, 2011, 5, 6048–6054.
Wang C., Wu J. & Mei X., Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding, Biotechnology Progress, 2001, 17, 89–94. DOI: https://doi.org/10.1021/bp0001359
Kim C.-H., Hong S.-H., Son S.-H. & Chung I.-S., Improved paclitaxel production in a two-phase suspension culture of Taxus cuspidata using silicone cubes, Biotechnology and Bioprocess Engineering, 1999, 4, 273–276. DOI: https://doi.org/10.1007/BF02933752
Sykłowska-Baranek K., Pilarek M., Bonfill M., Kafel K. & Pietrosiuk A., Perfluorodecalin-supported system enhances taxane production in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene, Plant Cell, Tissue and Organ Culture, 2015, 120, 1051–1059. DOI: https://doi.org/10.1007/s11240-014-0659-1
Krafft M. P., Fluorocarbons and fluorinated amphiphiles in drug delivery and biomedical research, Advanced Drug Delivery Reviews, 2001, 47, 209–228. DOI: https://doi.org/10.1016/S0169-409X(01)00107-7
Krafft M. P. & Riess J. G., Perfluorocarbons: Life sciences and biomedical uses dedicated to the memory of Professor Guy Ourisson, a true renaissance man, Journal of Polymer Science, Part A: Polymer Chemistry, 2007, 45, 1185–1198. DOI: https://doi.org/10.1002/pola.21937
Lowe K. C., Davey M. R. & Power J. B., Perfluorochemicals: Their applications and benefits to cell culture, Trends in Biotechnology, 1998, 16, 272–278. DOI: https://doi.org/10.1016/S0167-7799(98)01205-0
Pilarek M. & Szewczyk K. W., Effects of perfluorinated oxygen carrier application in yeast, fungi and plant cell suspension cultures, Biochemical Engineering Journal, 2008, 41, 38–42. DOI: https://doi.org/10.1016/j.bej.2008.03.004
Kanokwaree K. & Doran P. M., Application of membrane tubing aeration and perfluorocarbon to improve oxygen delivery to hairy root cultures, Biotechnology Progress, 1998, 14, 479–486. DOI: https://doi.org/10.1021/bp9800283
Pilarek M., Grabowska I., Senderek I., Wojasiński M., Janicka J., Janczyk-Ilach K. & Ciach T., Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds, Bioprocess and Biosystems Engineering, 2014, 37, 1707–1715. DOI: https://doi.org/10.1007/s00449-014-1143-3
Lowe K. C., Perfluorochemical respiratory gas carriers: Benefits to cell culture systems, Journal of Fluorine Chemistry, 2002, 118, 19–26. DOI: https://doi.org/10.1016/S0022-1139(02)00200-2
Touno K., Tamaoka J., Ohashi Y. & Shimomura K., Ethylene induced shikonin biosynthesis in shoot culture of Lithospermum erythrorhizon, Plant Physiology and Biochemistry, 2005, 43, 101–105. DOI: https://doi.org/10.1016/j.plaphy.2005.01.004
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
For all articles published in Prospects in Pharmaceutical Sciences copyright is retained by the authors. The journal publishes articles in the Open Access policy, within the CC BY 4.0 licence.
This is a human-readable summary of (and not a substitute for) the license. Disclaimer.
You are free to:
Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
How to Cite
