EKSTRAKCJA IN SITU ROŚLINNYCH METABOLITÓW WTÓRNYCH

Mateusz Kawka; Maciej Pilarek; Katarzyna Sykłowska-Baranek; Agnieszka Pietrosiuk

doi:10.56782/pps.78

Published : 2017-08-21

Vol. 15 No. 7 (2017)

IN SITU EXTRACTION OF PLANT SECONDARY METABOLITES

Mateusz Kawka

Maciej Pilarek

Katarzyna Sykłowska-Baranek

Agnieszka Pietrosiuk

DOI: https://doi.org/10.56782/pps.78

Abstract

Besides being a method to isolate plant metabolites from an in vitro culture, in situ extraction may enhance the biosynthesis of such metabolites in cultured plant biomass. In keeping with its definition, the process is based on the extraction of secondary metabolites, as they are being formed, via their accumulation in an additional phase of the culture system. Driven by the potential benefits attributable to this process, the search for in situ extractants and adsorbents that are non-toxic to the biomass and selective for the secondary metabolites yielded numerous candidate materials described in the scientific literature. A relatively new approach is the use of liquid perfluorochemicals (PFC) as the additional in situ extraction phase. Previously known as efficient carriers of gases, PFCs do not interact in any significant way with molecules dissolved in them. This results in a facile release of gases across the liquid-liquid interface of the culture system. Moreover, being immiscible with water, PFCs form in the presence of an aqueous culture medium a separate, lower phase. These properties of PFCs, in combination with a lack of toxicity toward living cells and a selectivity for plant metabolites, explain the mounting interest in the application of liquid perfluorochemicals as in situ extractants.

Keywords:

in situ extraction, two-phase cultures, liquid perfluorochemicals

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Kawka, M., Pilarek, M., Sykłowska-Baranek, K., & Pietrosiuk, A. (2017). IN SITU EXTRACTION OF PLANT SECONDARY METABOLITES. Prospects in Pharmaceutical Sciences, 15(7), 60–67. https://doi.org/10.56782/pps.78

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Mateusz Kawka

Affiliation:

Zakład Biotechnologii i Inżynierii Bioprocesowej, Wydział Inżynierii Chemicznej i Procesowej, Politechnika Warszawska, ul. Waryńskiego 1, 00-645 Warszawa; Zakład Biologii Farmaceutycznej i Biotechnologii Roślin Leczniczych, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej, Warszawski Uniwersytet Medyczny, ul. Banacha 1, 02-097 Warszawa Poland

Maciej Pilarek

Affiliation:

Zakład Biotechnologii i Inżynierii Bioprocesowej, Wydział Inżynierii Chemicznej i Procesowej, Politechnika Warszawska, ul. Waryńskiego 1, 00-645 Warszawa Poland

Katarzyna Sykłowska-Baranek

katarzyna.syklowska-baranek@wum.edu.pl

Affiliation:

Zakład Biologii Farmaceutycznej i Biotechnologii Roślin Leczniczych, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej, Warszawski Uniwersytet Medyczny, ul. Banacha 1, 02-097 Warszawa * autorka kore Poland

Agnieszka Pietrosiuk

Affiliation: