Determination of the antimicrobial, antioxidant activities and effects on oxidative DNA damage of extracts from three different Salvia species grown in Turkey

Mehmet Kaygısız; Eda Sönmez Gürer

doi:10.56782/pps.300

Published : 2025-01-15

Vol. 23 No. 1 (2025)

Determination of the antimicrobial, antioxidant activities and effects on oxidative DNA damage of extracts from three different Salvia species grown in Turkey

Mehmet Kaygısız

Eda Sönmez Gürer

DOI: https://doi.org/10.56782/pps.300

Abstract

Salvia officinalis L., known as medicinal sage, Salvia triloba L., known as Anatolian sage, and Salvia sclarea L., known as clary sage, belong to the Lamiaceae family and are species that typically grow in the Mediterranean region. This study aims to investigate the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) values, antioxidant and antimicrobial activities, total flavonoid and total phenolic content, and protective effects against oxidative DNA damage of extracts obtained from the aerial parts of three sage species — S. officinalis, S. triloba, and S. sclarea — which are cultivated using organic farming techniques in Turkey. Ready kits were used to determine TAS, TOS, and OSI values, while the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was used for antioxidant activity assessment. The broth microdilution method was used to determine the minimum inhibitory concentration (MIC) against microorganisms. To evaluate the protective effects of the extracts on DNA against UV and oxidative damage, pBR322 plasmid DNA was used. In conclusion, among the prepared water, 70% ethanol, and ethyl acetate extracts, the highest TAS was found in S. triloba 70% ethanol extract (9.6±1.8 mmol/L). In contrast, the highest TOS was observed in S. sclarea water extract (3.9±1.2 μmol/L). The OSI values of all plant species and extracts were found to be very similar. The extract with the highest antioxidant activity was S. officinalis 70% ethanol extract (IC₅₀= 0.105±0.056 mg/mL), and all extracts demonstrated low to moderate antimicrobial activity against the tested microorganisms. The highest total phenolic content was determined in S. triloba ethyl acetate extract (37.6±8.5 mg GAE/g extract), while the highest total flavonoid content was observed in S. officinalis ethyl acetate extract (12.5±2.9 mg QE/g extract). All extracts from the three plant species provided strong protection for plasmid DNA molecules against oxidative damage.

Keywords:

Sage, Antimicrobial, Antioxidant, DNA protective effect

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Kaygısız, M., & Sönmez Gürer, E. (2025). Determination of the antimicrobial, antioxidant activities and effects on oxidative DNA damage of extracts from three different Salvia species grown in Turkey. Prospects in Pharmaceutical Sciences, 23(1), 1–8. https://doi.org/10.56782/pps.300

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References

1. Gülçin, L.; Topal, F.; Beyza, S.; Sarıkaya, Ö.; Bursal, E.; Bilsel, G.; Gören, A.C. Polyphenol contents and antioxidant properties of Medlar (Mespilus Germanica L.). Rec. Nat. Prod. 2011, 5(3), 158-175.

2. Özdemir, E.; Alpınar, K. An ethnobotanical survey of medicinal plants in western part of central taurus mountains: Aladaglar (Nigde – Turkey). J. Ethnopharmacol. 2015, 166, 53–65. https://doi.org/10.1016/j.jep.2015.02.052

3. Rojas, R.; Bustamante, B.; Bauer, J.; Fernández, I.; Albán, J.; Lock, O. Antimicrobial activity of selected peruvian medicinal plants. J. Ethnopharmacol. 2003, 88 (3), 199–204. https://doi.org/10.1016/S0378-8741(03)00212-5

4. Ivanova, D.; Gerova, D.; Chervenkov, T.; Yankova, T. Polyphenols and antioxidant capacity of bulgarian medicinal plants. J. Ethnopharmacol. 2005, 96 (2), 145–150. https://doi.org/10.1016/j.jep.2004.08.033

5. Angerhofer, C.K. Sage: The genus Salvia. J. Nat. Prod. 2001, 64 (9), 1258–1258. https://doi.org/10.1021/np000756b

6. İpek, A.; Gürbüz, B. Türkiye florasında bulunan Salvia türleri ve tehlike durumları. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi. 2010, 1–2 (19), 30–35.

7. Ceylan, A. Ege üniversitesi ziraat fakültesi tarla bitkileri bölümünde tıbbi ve aromatik bitkiler üzerinde yapılan agronomik çalışmalar. In Tıbbi ve Aromatik Bitkiler Çalıştayı; Bornova-İzmir, 1995.

8. Ceylan, A.; Yurtseven, M.; Ozansoy, Y. Salvia officinalis L.’nin agronomik ve teknolojik özelliklerine azotlu gübrelemenin etkisi üzerinde araştırma. Ege Üniversitesi Ziraat Fakültesi Dergisi. 1979, 16 (3), 83–96.

9. Kırıcı, S.; Özgüven, M.; Yenikalaycı, A. Çukurova bölgesinde tıbbi adaçayı (Salvia officinalis L.) üzerinde araştırmalar. In Tıbbi ve Aromatik Bitkiler Çalıştayı; Bornova-İzmir, 1995; 39–40.

10. Topçu, G. Bioactive triterpenoids from Salvia species. J. Nat. Prod. 2006, 69 (3), 482–487. https://doi.org/10.1021/np0600402

11. Lu, Y.; Yeap Foo, L. Polyphenolics of Salvia—a review. Phytochemistry 2002, 59 (2), 117–140. https://doi.org/10.1016/S0031-9422(01)00415-0

12. Gürdal, B.; Kültür, Ş. An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). J. Ethnopharmacol. 2013, 146 (1), 113–126. https://doi.org/10.1016/j.jep.2012.12.012

13. Mata, A.T.; Proença, C.; Ferreira, A.R.; Serralheiro, M.L.M.; Nogueira, J.M.F.; Araújo, M.E.M. Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chem. 2007, 103 (3), 778–786. https://doi.org/10.1016/j.foodchem.2006.09.017

14. Erel, O. A Novel automated method to measure total antioxidant response against potent free radical reactions. Clin. Biochem. 2004, 37 (2), 112–119. https://doi.org/10.1016/j.clinbiochem.2003.10.014

15. Erel, O. A New automated colorimetric method for measuring total oxidant status. Clin. Biochem. 2005, 38 (12), 1103–1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008

16. Cuendet, M.; Hostettmann, K.; Potteratt, O. Iridoid glucosides with free radical scavening properties from Fagraea blumei. Helv. Chim. Acta. 1997, 80(4), 1144-1152. https://doi.org/10.1002/hlca.19970800411

17. Frankel, E.N.; Meyer, A.S. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J. Sci. Food Agric. 2000, 80 (13), 1925–1941. https://doi.org/10.1002/1097-0010(200010)80:13<1925

18. Eren, E. Bazi soğansi bitkilerin antioksidan aktivitelerinin belirlenmesi. University of Sakarya, Sakarya, 2011.

19. Chandra Shekhar, T.; Anju, G. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. Leaves, Shekhar, Tailor Chandra and Goyal Anju. Am. J. Ethnomed. 2014, 1, 244-249.

20. İzol, E. Phytochemicals in honey and health effects. In Honeybees. Plants And Health; Orient Publications, 2023; 85–96.

21. Dündar, E. Centaurea babylonica L. bitkisi üzerinde farmakognozik araştırmalar. University of Anadolu, Eskişehir, 2017.

22. Arıkan, H. Inula graveolens (L.) Desf. bitkisi üzerinde farmakognozik çalışmalar. University of Anadolu, Eskişehir, 2019.

23. Eloff, J. A sensitive and quick microplate method to determine the minimal ınhibitory concentration of plant extracts for bacteria. Planta Med. 1998, 64 (08), 711–713. https://doi.org/10.1055/s-2006-957563

24. Saraç, H.; Daştan, T.; Demirbaş, A.; Durna Daştan, S.; Karaköy, T.; Durukan, H. Madımak (Polygonum cognatum Meissn.) bitki özütlerinin besin elementleri ve in vitro antikanserojen aktiviteleri yönünden değerlendirilmesi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 1. Uluslararası Tarımsal Yapılar ve Sulama Kongresi Özel Sayısı. 2018, 340–347.

25. Saraç, H.; Daştan, T.; Durukan, H.; Durna Daştan, S.; Demirbaş, A.; Karaköy, T. Kırmızı gelincik (Fam: Papaveraceae, Glaucium grandiflorum Boiss.&Huet Var. Grandiflorum) bitkisinin farklı özütlerinin besin elementi ıçeriğinin ve in vitro antiproliferatif etkilerinin değerlendirilmesi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 1. Uluslararası Tarımsal Yapılar ve Sulama Kongresi Özel Sayısı. 2018, 417–428.

26. Barut Uyar, B.; Gezmen Karadağ, M.; Şanlıer, N.; Günyel, S. Toplumumuzda sıklıkla kullanılan bazı bitkilerin toplam fenolik madde miktarlarının saptanması. Gıda. 2013, 1 (38), 23–29.

27. Demir, N.; Yildiz, O.; Alpaslan, M.; Hayaloglu, A.A. Evaluation of volatiles, phenolic compounds and antioxidant activities of Rose hip (Rosa L.) fruits in Turkey. LWT-Food Sci. Technol. 2014, 57 (1), 126–133. https://doi.org/10.1016/j.lwt.2013.12.038.

28. Gökgöz, Y. Burdur ıli pazarlarında satılan bazı meyvelerin antioksidan kapasitelerinin belirlenmesi. Mehmet Akif Ersoy Üniversitesi, Burdur. 2015.

29. Cemeroğlu, B. Gıda analizleri; Gıda Teknolojisi Derneği Yayınları, Ankara. 2007.

30. Fersahoğlu, H. Farklı renklerdeki gülhatmi çiçeklerinin biyoaktif özellikleri. Yıldız Teknik Üniversitesi, İstanbul. 2016.

31. Yıldız, İ. Türkiye’de yetişen bazı bitki türlerinin antimikrobiyal ve antioksidan özelliklerinin ıncelenmesi. Anadolu Üniversitesi, Eskişehir. 2019.

32. Sönmez Gürer, E. Pharmacognosic studies on Aronia melanocarpa (Michx) Elliot species. University of Anadolu, Eskişehir. 2022.

33. Dewanto, V.; Wu, X.; Adom, K.K.; Liu, R.H. Thermal processing enhances the nutritional value of tomatoes by ıncreasing total antioxidant activity. J. Agric. Food Chem. 2002, 50 (10), 3010–3014. https://doi.org/10.1021/jf0115589

34. Aydın, G. Lupinus angustifolius L. Subsp. Angustifolius (Fabaceae) taksonunun antioksidan, anti-tüberküloz ve antifungal aktivitelerinin belirlenmesi. Balıkesir Üniversitesi, Balıkesir. 2021.

35. Chang, C.; Yang, M.; Wen, H.; Chern, J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 2002, 10, 178–182.

36. Gajula, D.; Verghese, M.; Boateng, J.; Walker, L.T.; Shackelfor, L.; Mentreddy, S.R.; Cedric, S. Determination of total phenolics, flavonoids and antioxidant and chemopreventive potential of Basil (Ocimum basilicum L. and Ocimum tenuiflorum L.). Int. J. Cancer Res. 2009, 5 (4), 130–143. https://doi.org/10.3923/ijcr.2009.130.143

37. İçli, N. Determination of total phenolic compounds, total antioxidant capacity and total flavonoid compounds in apple sour. Sağlık Akademisi Kastamonu. 2017, 2 (2), 89–99.

38. Russo, A.; Acquaviva, R.; Campisi, A.; Sorrenti, V.; Di Giacomo, C.; Virgata, G.; Barcellona, M.L.; Vanella, A. Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors. Cell. Biol. Toxicol. 2000, 16 (2), 91–98. https://doi.org/10.1023/A:1007685909018

39. Berk, Ş. Myrtus communis, Pistacia vera, Arum maculatum, Ceterach officinarum, Inula oculus-christi türlerinin antioksidan, anti-mikrobiyal ve dna koruyucu aktivitelerinin araştırılması. Cumhuriyet Üniversitesi, Sivas. 2012.

40. Takım, K. Kiraz yaprağı ekstraktlarının antioksidan kapasitesinin ve oksidatif dna hasarı üzerine etkisinin belirlenmesi. İnönü Üniversitesi, Malatya. 2010.

41. Kuete, V. Potential of Cameroonian plants and derived products against microbial ınfections. Planta Med. 2010, 76, 1479–1491. http://dx.doi.org/10.1055/s-0030-1250027

42. Awouafack, M.D.; McGaw, L.J.; Gottfried, S.; Mbouangouere, R.; Tane, P.; Spiteller, M.; Eloff, J.N. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds ısolated from Eriosema robustum (Fabaceae). BMC Complement. Altern. Med. 2013, 13, Art. No: 289. https://doi.org/10.1186/1472-6882-13-289

43. Yadav, S.; Mukundan, U. In vitro antioxidant properties of Salvia coccinea Buc’hoz Ex Etl. and Salvia officinalis L. Indian J. Fundam. Appl. Life Sci. 2011, (1), 3, 232-238.

44. Gülçi̇n, I.; Uğuz, M.T.; Oktay, M.; Beydemi̇r, Ş.; İrfan Küfrevi̇oğlu, Ö. Evaluation of the antioxidant and antimicrobial activities of Clary Sage (Salvia sclarea L.). Turk. J. Agri. For. 2004, 28(1), Article No: 4. Available at: https://journals.tubitak.gov.tr/agriculture/vol28/iss1/4

45. Yücel, Y.Y.; Özdemir, E. Determination of biological activities of Salvia sclarea L. Fabad J. Pharm. Sci. 2023, 48(2), 255-264. https://doi.org/10.55262/fabadeczacilik.1293118

46. Özüpek, B.; Pekacar, S.; Deliorman Orhan, D. Evaluation of phytochemical contents and biological activities of Salvia officinalis and Salvia triloba grown with organic farming. Fabad J. Pharm. Sci. 2022, 48(1), 125 – 138. https://doi.org/10.55262/fabadeczacilik.1175781

47. Nickavar, B.; Kamalinejad, M.; Izadpanah, H. In vitro free radical scavenging activity of five Salvia specıes. Pak. J. Pharm. Sci. 2007, 20 (4), 291-294.

48. Horiuchi, K.; Shiota, S.; Hatano, T.; Yoshida, T.; Kuroda, T.; Tsuchiya, T. Antimicrobial activity of oleanolic acid from Salvia officinalis and related compounds on vancomycin-resistant enterococci (VRE). Biol. Pharm. Bull. 2007, 30 (6), 1147–1149. https://doi.org/10.1248/bpb.30.1147

49. Abdelkader, M.; Ahcen, B.; Rachid, D.; Hâkim, H. Phytochemical study and biological activity of Sage (Salvia officinalis L.). World Academy of Science, Engineering and Technology International Journal of Bioengineering and Life Sciences 2014, 8 (11), 1254-1257.

50. Ramos, A.A.; Azqueta, A.; Pereira-Wilson, C.; Collins, A. R. Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cells. J. Agric. Food Chem. 2010, 58 (12), 7465–7471. https://doi.org/10.1021/jf100082p

51. Hani, S.B.; Bayachou, M. Salvia fruticosa reduces ıntrinsic cellular and H2O2-Induced DNA oxidation in HEK 293 cells; assessment using flow cytometry. Asian Pac. J. Trop. Biomed. 2014, 4 (5), 399–403. https://doi.org/10.12980/APJTB.4.2014C1270