Antioxidant potential and fatty acid composition of Lactarius sanguifluus

Sükrü Canpolat; Cihan Düşgün; Elif Ildız; Elif Yürümez Canpolat; Cemil İşlek

doi:10.56782/pps.225

Published : 2024-10-03

Vol. 22 No. 4 (2024)

Antioxidant potential and fatty acid composition of Lactarius sanguifluus

Sükrü Canpolat

Cihan Düşgün

Elif Ildız

Elif Yürümez Canpolat

Cemil İşlek

DOI: https://doi.org/10.56782/pps.225

Abstract

Edible mushrooms have been valued as a source of food and traditional medicines since ancient times. Mushrooms have drawn the attention of numerous researchers in recent decades since they contain a variety of bioactive compounds with antibacterial, antiallergic, antidiabetic, antitumoral, and antioxidant activities. As a member of the Russulaceae family Lactarius sanguifluus (Paulet) Fr. (1838) (also known as bloody milk cap) is an ectomycorrhizal edible mushroom distributed in Asia, the Mediterranean shores and mid-Europe. In this study it was aimed to identify the mushroom sample both by physical traits according to the current literature and by molecular techniques. The antioxidant capability of L. sanguifluus methanol extract was evaluated by stable radical scavenging activity and metal ion reducing capability methods. The content of total phenolic compounds and the fatty acid composition of the mushroom sample were also investigated. The mushroom sample was identified both by comparing the physical traits with the current literature and by comparing the sequence data of the ITS region of rDNA with GenBank. The methanol extract of the dried mushroom sample was used to perform the analysis to determine the antioxidant activity and content of total phenolic compounds. It was found that L. sanguifluus methanol extract contained 3.677±0.207 mg GAE/g DW phenolic compounds and exhibited radical scavenging activities as 211.652±0.537 mg TE/g DW against DPPH and 73.389±1.003 mg TE/g DW against ABTS. L. sanguifluus methanol extract also had metal ion reducing capabilities with ferric reducing and cupric reducing antioxidant capacities of 191.903±0.659 mg AAE/g DW and 379.598±7.527 mg TE/g DW, respectively. The GC-MS analysis of the hexane extract of the mushroom sample revealed that palmitic acid, linoleic acid, oleic acid and stearic acid were the predominant fatty acids found in L. sanguifluus.

Keywords:

Lactarius sanguifluus, Molecular identification, Antioxidant activity, Total phenolics, Fatty acids

Similar Articles

Sylwester Krukowski, SYNTHESIS AND STRUCTURAL STUDIES OF CALCIUM HYDROXYAPATITES MODIFIED WITH AMINO ACIDS , Prospects in Pharmaceutical Sciences: Vol. 11 No. 7 (2013)
Anna Babis, USABILITY OF THE FRUITS OF ROSA SPINOSISSIMA AND ROSA HYBRIDA TO THE PRODUCTION OF TURBID MULTIVITAMIN JUICES , Prospects in Pharmaceutical Sciences: Vol. 2 No. 3 (2004)
Krzysztof Kubica, Aleksander Mazurek, VEMURAFENIB AS A SELECTIVE INHIBITOR OF THE SERINE/THREONINE-PROTEIN KINASE B-RAF, USED IN THE TREATMENT OF MELANOMA , Prospects in Pharmaceutical Sciences: Vol. 14 No. 8 (2016)
Zofia Suchocka, OBESITY - REASONS AND TREATMENT , Prospects in Pharmaceutical Sciences: Vol. 1 No. 1 (2003)
Magdalena Bamburowicz-Klimkowska, Mirosław M. Szutowski, STRATEGIES TO COMBAT MULTIDRUG RESISTANCE OF CANCER , Prospects in Pharmaceutical Sciences: Vol. 10 No. 1 (2012)
Aleksandra Kowalska, The Vitamin E: overview of history of discovery, mechanism of action, role and deficiency , Prospects in Pharmaceutical Sciences: Vol. 22 No. 3 (2024)
Ewa Napiórkowska, Thiamine: overview of history of discovery, mechanism of action, role and deficiency , Prospects in Pharmaceutical Sciences: Vol. 21 No. 3 (2023)
Teresa Żołek, Aleksandra Trzeciak, THE MECHANISM OF ACTION OF INDOLE-3-CARBINOL AND 3,3´-DIINDOLYLMETHANE IN CANCER CHEMOPREVENTION , Prospects in Pharmaceutical Sciences: Vol. 15 No. 2 (2017)
Daria Żuraw, Paulina Oleksa, Mateusz Sobczyk, Kacper Jasiński, Simple obesity in children , Prospects in Pharmaceutical Sciences: Vol. 21 No. 2 (2023)
Natalia Cichoń, Dominika Lach, Joanna Saluk-Bijak, Michał Bijak, ANTICOAGULANT THERAPY IN ACUTE CORONARY SYNDROMES AND ISCHEMIC STROKE , Prospects in Pharmaceutical Sciences: Vol. 15 No. 5 (2017)

<< < 1 2 3 4 5 6 7 8 > >>

You may also start an advanced similarity search for this article.

Details

References

Statistics

Authors

Download files

PDF

Citation rules

Canpolat, S., Düşgün, C., Ildız, E., Yürümez Canpolat, E., & İşlek, C. (2024). Antioxidant potential and fatty acid composition of Lactarius sanguifluus. Prospects in Pharmaceutical Sciences, 22(4), 1–6. https://doi.org/10.56782/pps.225

Altmetric indicators

Cited by / Share

Licence

This work is licensed under a Creative Commons Attribution 4.0 International License.

References

Webster, J.; Weber, R. Introduction to Fungi, 3rd ed.; Cambridge University Press: UK, 2007; pp. 1-39. DOI: https://doi.org/10.1017/CBO9780511809026

Ildız, E.; Canpolat, Ş.; İşlek, C.; Canpolat, E.Y.; İşlek, Y.; Akata, I. Bjerkandera adusta collected from Niğde: analysis of total phenolic compound, antioxidant, and antimicrobial properties. Turkish JAF Sci. Tech. 2022, 10, 2996-3000. DOI: 10.24925/turjaf.v10isp2.2996-3000.5750 DOI: https://doi.org/10.24925/turjaf.v10isp2.2996-3000.5750

Kosanic, M.; Petrović, N.; Milosevic-Djordjevic, O.; Grujičić, D.; Tubic, J.; Marković, A.; Stanojkovic, T.P. The health promoting effects of the fruiting bodies extract of the peppery milk cap mushroom Lactarius piperatus (Agaricomycetes) from Serbia. Int. J. Med. Mushrooms 2020, 22(4), 347–357. DOI: 10.1615/IntJMedMushrooms.2020034167 DOI: https://doi.org/10.1615/IntJMedMushrooms.2020034167

Barros, L.; Venturini, B.A.; Baptista, P.; Estevinho, L.M.; Ferreira, I.C.F.R. Chemical composition and biological properties of Portuguese wild mushrooms: a comprehensive study. J. Agric. Food Chem. 2008, 56(10), 3856-3862. DOI: 10.1021/jf8003114 DOI: https://doi.org/10.1021/jf8003114

Kumar, K.; Mehra, R.; Guiné, R.P.; Lima, M.J.; Kumar, N.; Kaushik, R.; Ahmed, N.; Yadav, A.N.; Kumar, H. Edible mushrooms: A comprehensive review on bioactive compounds with health benefits and processing aspects. Foods 2021, 10(12), 2996-3006. DOI: 10.3390/foods10122996 DOI: https://doi.org/10.3390/foods10122996

Yim, H.S.; Chye, F.Y.; Tan, C.T.; Ng, Y.C.; Ho, C.W. Antioxidant activities and total phenolic content of aqueous extract of Pleurotus ostreatus (cultivated oyster mushroom). Malays. J. Nutr. 2010, 16(2), 281-291.

Fu, H.Y.; Shieh, D.E.; Ho, C.T. Antioxidant and free radical scavenging activities of edible mushrooms. J. Food Lipids 2002, 9(1), 35-43. DOI: 10.1111/j.1745-4522.2002.tb00206.x DOI: https://doi.org/10.1111/j.1745-4522.2002.tb00206.x

Rustan, A.C. and Drevon, C.A. Fatty acids: structures and properties. Proc. Natl. Acad. Sci. 2012, 109(16), 6241–6246. DOI: 10.1038/npg.els.0003894 DOI: https://doi.org/10.1038/npg.els.0003894

Sevindik, M.; Akgül, H.; Bal, C. Determination of oxidative stress status of Ompholatus olearius gathered from Adana and Antalya provinces in Turkey. SAUJS 2017, 21(3), 324-327. DOI: 10.16984/saufenbilder.298974 DOI: https://doi.org/10.16984/saufenbilder.09547

Bal, C.; Akgul, H.; Sevindik, M.; Akata, I.; Yumrutas, O. Determination of the anti-oxidative activities of six mushrooms. Fresenius Environ. Bull. 2017, 26(10), 6246-6252.

Breitenbach, J.; Kränzlin, F. Fungi of Switzerland,2nd ed.; Verlag Mykologia: Lucerna, Switzerland, 1986.

Cannon, P.F. and Kirk, P.M. Fungal Families of the World, 1st ed.; CABI: Netherland, 2007. DOI: https://doi.org/10.1079/9780851998275.0000

Hansen, L. and Knudsen, H. Nordic Macromycetes: Polyporales, Boletales, Agaricales, Russulales, Nordsvamp: Copenhagen, 1992.

Schoch, C.L.; Seifert, K.A.; Huhndorf, S.; Robert, V.; Spouge, J.L.; Levesque, C.A.; Chen, W.; Consortium, F.B.; List, F.B.C.A.; Bolchacova, E. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc. Natl. Acad. Sci. 2012, 109(16), 6241-6246. DOI: 10.1073/pnas.1117018109 DOI: https://doi.org/10.1073/pnas.1207508109

White, T.J.; Bruns, T.; Lee, S.J.W.T.; Taylor, J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., Eds., Academic Press: New York, 1990, 18(1), 315-322. DOI: https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Singleton, V.L.; R, O.; Lamuela-Raventós, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999, 299, 152-178. DOI: 10.1016/S0076-6879(99)99017-1 DOI: https://doi.org/10.1016/S0076-6879(99)99017-1

Shimada, K.; Fujikawa, K.; Yahara, K.; Nakamura, T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem. 1992, 40(6), 945-948. DOI: doi.org/10.1021/jf00018a005 DOI: https://doi.org/10.1021/jf00018a005

Blois, M.S. Antioxidant determinations by the use of a stable free radical. Nature 1958, 181(4617), 1199-1200. DOI: 10.1038/1811199a0 DOI: https://doi.org/10.1038/1811199a0

Cam, M.; Basyigit, B.; Alasalvar, H.; Yilmaztekin, M.; Ahhmed, A.; Sagdic, O.; Konca, Y.; Telci, I. Bioactive properties of powdered peppermint and spearmint extracts: Inhibition of key enzymes linked to hypertension and type 2 diabetes. Food Biosci. 2020, 35, Art. No: 100577. DOI: 10.1016/j.fbio.2020.100577 DOI: https://doi.org/10.1016/j.fbio.2020.100577

Xiao, F.; Xu, T.; Lu, B.; Liu, R. Guidelines for antioxidant assays for food components. Food Front. 2020, 1(1), 60-69. DOI: 10.1002/fft2.10 DOI: https://doi.org/10.1002/fft2.10

Mokrani, A.; Krisa, S.; Cluzet, S.; Da Costa, G.; Temsamani, H.; Renouf, E.; Mérillon, J.-M.; Madani, K.; Mesnil, M.; Monvoisin, A. Phenolic contents and bioactive potential of peach fruit extracts. Food Chem. 2016, 202, 212-220. DOI: 10.1016/j.foodchem.2015.12.026 DOI: https://doi.org/10.1016/j.foodchem.2015.12.026

Düşgün, C.; Kankılıç, T.; İşlek, C.; Balı, D.F.; Kankılıç, Ö. Antioxidant and cytotoxic potential of local endemic plant Pastinaca zozimoides Fenzl. Turkish JAF Sci. Tech. 2021, 9(4), 646-649. DOI: 10.24925/turjaf.v9i4.646-649.3715 DOI: https://doi.org/10.24925/turjaf.v9i4.646-649.3715

Apak, R.; Güçlü, K.; Özyürek, M.; Esin Karademir, S.; Erçağ, E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int. J. Food Sci. Nutr. 2006, 57(5/6), 292-304 DOI: 10.1080/09637480600798132. DOI: https://doi.org/10.1080/09637480600798132

Canpolat, Ş. and Canpolat, E.Y. Antioxidant and Antimicrobial Activity of a Medicinal Mushroom, Ganoderma lucidum. J. Adv. Biol. Biotechnol. 2023, 26(11), 60-67. DOI: 10.9734/jabb/2023/v26i11667 DOI: https://doi.org/10.9734/jabb/2023/v26i11667

Erbiai, E.H.; Bouchra, B.; da Silva, L.P.; Lamrani, Z.; Pinto, E.; da Silva, J.C.E.; Maouni, A. Chemical composition and antioxidant and antimicrobial activities of Lactarius sanguifluus, a wild edible mushroom from northern Morocco. Euro-Mediterr. J. Environ. Integr. 2021, 6, 1-12. DOI:10.1007/s41207-021-00247-6 DOI: https://doi.org/10.1007/s41207-021-00247-6

Kalogeropoulos, N.; Yanni, A.E.; Koutrotsios, G.; Aloupi, M. Bioactive microconstituents and antioxidant properties of wild edible mushrooms from the island of Lesvos, Greece. Food Chem. Toxicol. 2013, 55, 378-385.DOI: 10.1016/j.fct.2013.01.010 DOI: https://doi.org/10.1016/j.fct.2013.01.010

Sharma, S.; Atri, N.S.; Kaur, M.; Verma, B. Nutritional and neutraceutical potential of some wild edible Russulaceous mushrooms from North West Himalayas, India. Kavaka 2017, 48, 41-46.

Emsen, B.; Guven, B.; Uzun, Y.; Kaya, A. Antioxidant and genotoxic effects of aqueous and methanol extracts from two edible mushrooms from Turkey in human peripheral lymphocytes. Int. J. Med. Mushrooms 2020, 22(2), 1-6. DOI: 10.1615/IntJMedMushrooms.2020033845 DOI: https://doi.org/10.1615/IntJMedMushrooms.2020033845

Yıldız, S. and Gürgen, A. Bazı yenilebilir yabani Lactarius mantarlarının biyoaktif ve radyoaktif özellikleri. Orman. Araşt. Derg. 2022, 9(Özel Sayı), 254-263. DOI: 10.17568/ogmoad.1111983 DOI: https://doi.org/10.17568/ogmoad.1111983

Erdoğan, S.; Soylu, M.K.; Başer, K.H.C. Bazı yabani mantarların antioksidan özellikleri. Nevşehir Bil. Teknol. Derg. 2017, 6, 254-260. DOI: 10.17100/nevbiltek.334595 DOI: https://doi.org/10.17100/nevbiltek.334595

Stanković, M.; Mitić, V.; Stankov Jovanović, V.; Dimitrijević, M.; Nikolić, J.; & Stojanović, G. (2022). Selected fungi of the genus Lactarius-screening of antioxidant capacity, antimicrobial activity, and genotoxicity. J. Toxicol. Env. Heal. A, 85(17), 699-714. DOI: 10.1080/15287394.2022.2075502 DOI: https://doi.org/10.1080/15287394.2022.2075502

Bonanome, A. and Grundy, S.M. Effect of dietary stearic acid on plasma cholesterol and lipoprotein levels. N. Engl. J. Med. 1988, 318(19), 1244-1248. DOI: 10.1056/NEJM198805123181905 DOI: https://doi.org/10.1056/NEJM198805123181905

Williams, C.M. Dietary fatty acids and human health. Ann. Zootech. 2000, 49(3), 165-180. DOI: 10.1051/animres:2000116 DOI: https://doi.org/10.1051/animres:2000116

Kalač, P. Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food Chem. 2009, 113(1), 9-16. DOI: 10.1016/j.foodchem.2008.07.077 DOI: https://doi.org/10.1016/j.foodchem.2008.07.077

Üstün, O. Makrofungusların besin değeri ve biyolojik etkileri. Türk Hij. Deney.Biyol. Derg. 2011, 68(4), 223-240. DOI: : 10.5505/TurkHijyen.2011.00922 DOI: https://doi.org/10.5505/TurkHijyen.2011.00922

Yılmaz, H.Ç. and Bengü, A.Ş. The ınvestigation of fatty acids and mineral profiles of some edible Lactarius species (L. deliciosus, L. deterrimus, L. salmonicolor, L. sanguifluus, L. semisanguifluus) in the Uşak/Turkey province of Aegean Region. Biol. Divers. Conserv. 2018, 11(1), 95-104.

Kostić, M.; Ivanov, M.; Fernandes, Â.; Calhelha, R.C.; Glamočlija, J.; Barros, L.; Soković, M.; Ćirić, A. A comparative study of Lactarius mushrooms: Chemical characterization, antibacterial, antibiofilm, antioxidant and cytotoxic activity. J. Fungi 2023, 9(1), 70-79. DOI: 10.3390/jof9010070 DOI: https://doi.org/10.3390/jof9010070

Sükrü Canpolat

sukrucanpolat@gmail.com
https://orcid.org/0000-0001-8223-7260

Affiliation:

Nigde Omer Halisdemir University Turkey

Bio Statement (e.g., department and rank)

Central Research Laboratory Center

Dr.

Cihan Düşgün

cihandusgun@ohu.edu.tr
https://orcid.org/0000-0003-2796-8356

Affiliation:

Niğde Ömer Halisdemir University Turkey

Bio Statement (e.g., department and rank)

Faculty of Science, Department of Biology

Asst. Prof. Dr.

Elif Ildız

elifildiz4@gmail.com
https://orcid.org/0000-0003-3080-3384

Affiliation:

Niğde Ömer Halisdemir University Turkey

Bio Statement (e.g., department and rank)

MSc. Candidate

Elif Yürümez Canpolat

elif.yurumez@ohu.edu.tr
https://orcid.org/0000-0003-1470-1169

Affiliation:

Niğde Ömer Halisdemir University Turkey

Bio Statement (e.g., department and rank)

Faculty of Science, Department of Biotechnology

Asst. Prof. Dr.

Cemil İşlek

cislek@ohu.edu.tr
https://orcid.org/0000-0002-6690-2846

Affiliation:

Niğde Ömer Halisdemir University Turkey

Bio Statement (e.g., department and rank)

Faculty of Science, Department of Biotechnology

Prof. Dr.