Evaluation of antioxidant potential, anticholinesterase activity and element content of Chlorophyllum rhacodes species
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https://doi.org/10.56782/pps.521Keywords:
Chlorophyllum rhacodes, antioxidant activity, anticholinesterase, heavy metal, elemental analysisAbstract
In this study, antioxidant potential, anticholinesterase activity, and elemental contents of Chlorophyllum rhacodes were evaluated. In order to determine the antioxidant capacity, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), DPPH radical scavenging activity, and FRAP ferric reducing power analyses were applied. According to the obtained results, the TAS value was determined as 3.125±0.060 mmol/L, the TOS value as 6.821±0.062 µmol/L, the OSI value as 0.218±0.003, DPPH activity as 55.430±1.438 mg TE/g, and the FRAP value as 72.540±1.461 mg TE/g. These data revealed that C. rhacodes showed a significant level of antioxidant activity. In the evaluation of anticholinesterase activity, inhibitory effects against AChE and BChE enzymes were determined, and IC₅₀ values were found as 94.330±1.536 µg/mL and 128.397±1.556 µg/mL, respectively. These results show that C. rhacodes exhibits limited inhibitory effect on these enzymes but may have a certain level of anticholinesterase potential. According to the results of elemental analysis, Cd (14.78±1.53), Cr (33.17±1.42), Mn (7.35±0.52), Cu (155.77±2.34), Fe (469.30±15.75), Pb (6.98±0.54), Ni (3.66±0.31) and Zn (76.18±2.25) mg/kg levels were detected in mushroom samples. In particular, it was observed that Cd and Cu levels were above the upper limits specified in the literature. This finding shows that C. rhacodes tends to accumulate some heavy metals and may be highly sensitive to environmental pollution. In conclusion, C. rhacodes exhibits a biologically remarkable profile with certain antioxidant and anticholinesterase activities and can also be considered as a potential indicator species in the biological monitoring of environmental metal exposure.
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1. Eraslan, E.C.; Altuntaş, D.; Baba, H.; Bal, C.; Akgül, H.; Akata, I.; Sevindik, M. Some biological activities and element contents of ethanol extract of wild edible mushroom Morchella esculenta. Sigma J. Eng. Nat. Sci. 2021, 39(1), 24–28.
2. Ahmad, M.; Alsayegh, A.; Ahmad, F.; Akhtar, M.; Alavudeen, S.; Bantun, F.; Wahab, S.; Ahmed, A.; Ali, M.; Elbendary, E.; Raposo, A.; Kambal, N.; Abdelrahman, M. Ganoderma lucidum: Insight into antimicrobial and antioxidant properties with development of secondary metabolites. Heliyon 2024, 10(3), Art. No: e25607. DOI: 10.1016/j.heliyon.2024.e25607
3. Çömlekçioğlu, U.; Özköse, E.; Akyol, I.; Ekinci, M.S. Fatty acid analysis of anaerobic ruminal fungi Neocallimastix, Caecomyces and Orpinomyces. Int. J. Agric. Biol. 2010, 12, 635–637. DOI: 10.1016/j.compag.2010.03.005
4. Sevindik, M.; Akgül, H.; Akata, I.; Alli, H.; Selamoğlu, Z. Fomitopsis pinicola in healthful dietary approach and their therapeutic potentials. Acta Aliment. 2017, 46(4), 464–469. DOI: 10.1556/066.2017.46.4.9
5. Sarıdoğan, B.G.O.; İşlek, C.; Baba, H.; Akata, I.; Sevindik, M. Antioxidant, antimicrobial, oxidant and element contents of Xylaria polymorpha and X. hypoxylon (Xylariaceae). Fresen. Environ. Bull. 2021, 30(05), 5400–5404.
6. Sevindik, M.; Bal, C.; Eraslan, E.C.; Uysal, I.; Mohammed, F.S. Medicinal mushrooms: A comprehensive study on their antiviral potential. Prospects Pharm. Sci. 2023, 21(2), 42–56. DOI: 10.56782/pps.141
7. Fogarasi, M.; Nemeș, S.; Fǎrcaș, A.; Socaciu, C.; Semeniuc, C.; Socaciu, M.; Socaci, S. Bioactive secondary metabolites in mushrooms: A focus on polyphenols, their health benefits and applications. Food Biosci. 2024, 56, Art. No: 105166. DOI: 10.1016/j.fbio.2024.105166
8. Sevindik, M.; Rasul, A.; Hussain, G.; Anwar, H.; Zahoor, M.; Sarfraz, I.; Selamoğlu, Z. Determination of anti-oxidative, anti-microbial activity and heavy metal contents of Leucoagaricus leucothites. Pak. J. Pharm. Sci. 2018, 31 (5(Suppl)), 1981–1986.
9. Tan, Y.; Mo, J.; Wang, Y.; Zhang, W.; Jiang, Y.; Xu, K.; Tan, G.; Liu, S.; Li, J.; Wang, W. The ethnopharmacology, phytochemistry and pharmacology of the genus Hericium. J. Ethnopharmacol. 2023, 319(Pt 3), Art. No: 117353. DOI: 10.1016/j.jep.2023.117353
10. Anusiya, G.; Prabu, G.; Yamini, N.; Sivarajasekar, N.; Rambabu, K.; Bharath, G.; Banat, F. A review of the therapeutic and biological effects of edible and wild mushrooms. Bioengineered 2021, 12(2), 11239–11268. DOI: 10.1080/21655979.2021.2001183
11. Bhambri, A.; Srivastava, M.; Mahale, V.; Mahale, S.; Karn, S. Mushrooms as potential sources of active metabolites and medicines. Front. Microbiol. 2022, 13, Art. No: 837266. DOI: 10.3389/fmicb.2022.837266
12. Kumaresan, V.; Sariha, C.; Murali, T.; Senthilarasu, G. Occurrence of gilled fungi in Puducherry, India. J. Threat. Taxa 2021, 13, 18878–18887. DOI: 10.11609/jott.6978.13.7.18878-18887
13. Sahu, K.; Pradhan, P.; Chandrawanshi, N. Revaluation of mushroom edibility based on differences in morphological characteristic. NewBioWorld 2023, 4(2), 15-17. DOI: 10.52228/nbw-jaab.2022-4-2-3
14. Fraser, D. Discussion of development processes in insect‐fungus association derived from the shaggy parasol fruiting on the nests of hairy wood ants. Ecol. Evol. 2019, 9(20), 11619–11630. DOI: 10.1002/ece3.5611
15. Grodzinskaya, A.; Samchuk, A.; Nebesnyi, V.; Honchar, H. Radiocesium (137Cs) and mineral elements in culinary-medicinal mushrooms from the southern outskirts of Kyiv, Ukraine. Int. J. Med. Mushrooms 2019, 21(1), 71–77. DOI: 10.1615/IntJMedMushrooms.2018029583
16. Šíma, J.; Vondruška, J.; Svoboda, L.; Šeda, M.; Rokos, L. The accumulation of risk and essential elements in edible mushrooms Chlorophyllum rhacodes, Suillus grevillei, Imleria badia, and Xerocomellus chrysenteron growing in the Czech Republic. Chem. Biodivers. 2019, 16(2), Art. No: e1800478. DOI: 10.1002/cbdv.201800478
17. Keskin, F.; Sarıkürkçü, C.; Demirak, A.; Akata, I.; Tepe, A. Wild mushrooms from Ilgaz Mountain National Park (Western Black Sea, Turkey): Element concentrations and their health risk assessment. Environ. Sci. Pollut. Res. 2022, 29(21), 31923–31942. DOI: 10.1007/s11356-021-18011-2
18. Todorović, J.; Vesić, A.; Petrović, N.; Kosanić, M. Antimicrobial potential of mushrooms Macrolepiota procera and Chlorophyllum rhacodes. Book Proc. ICCBI 2023, 304t. DOI: 10.46793/iccbi23.304t
19. Galland, J.; Bourdic, F.; Yaouanc, B.; Piou, T.; Maurizi, A.; Deluegue, S. Comment on “Got milk? A case series of an amatoxin-exposed family, including a breastfeeding mother and infant”. Clin. Toxicol. 2021, 59(8), Art. No: 770. DOI: 10.1080/15563650.2020.1865544
20. Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 2004, 37(4), 277–285. DOI: 10.1016/j.clinbiochem.2003.11.015
21. Erel, O. A new automated colorimetric method for measuring total oxidant status. Clin. Biochem. 2005, 38(12), 1103–1111. DOI: 10.1016/j.clinbiochem.2005.08.008
22. Sevindik, M. Anticancer, antimicrobial, antioxidant and DNA protective potential of mushroom Leucopaxillus gentianeus (Quél.) Kotl. Indian J. Exp. Biol. 2021, 59, S310–S315.
23. Sevindik, M.; Gürgen, A.; Krupodorova, T.; Uysal, İ.; Koçer, O. A hybrid artificial neural network and multi-objective genetic algorithm approach to optimize extraction conditions of Mentha longifolia and biological activities. Sci. Rep. 2024, 14(1), Art. No: 31403. DOI: 10.1038/s41598-024-83029-8
24. Ellman, G.L.; Courtney, K.D.; Andres, V.; Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961, 7, 88–95. DOI: 10.1016/0006-2952(61)90145-9
25. Baba, H.; Sevindik, M.; Doğan, M.; Akgül, H. Antioxidant, antimicrobial activities and heavy metal contents of some Myxomycetes. Fresen. Environ. Bull. 2020, 29(9), 7840–7846.
26. İşlek, C.; Sarıdoğan, B.G.Ö.; Sevindik, M.; Akata, I. Biological activities and heavy metal contents of some Pholiota species. Fresen. Environ. Bull. 2021, 30(06), 6109–6114.
27. Xia, Y.; Wang, D.; Li, J.; Chen, M.; Wang, D.; Jiang, Z.; Liu, B. Compounds purified from edible fungi fight against chronic inflammation through oxidative stress regulation. Front. Pharmacol. 2022, 13, Art. No: 974794. DOI: 10.3389/fphar.2022.974794
28. Çolak, S.; Çömlekçioğlu, N.; Aygan, A.; Kocabaş, Y.Z.; Çömlekçioğlu, U. Phytochemical properties and bioactive potential of various Astragalus spp. from Turkey. Food Biosci. 2025, 64, Art. No: 105901. DOI: 10.1016/j.fbio.2025.105901
29. Abdelkader, M.; Mediatrice, H.; Lin, D.; Lin, Z.; Aggag, S. Mitigating oxidative stress and promoting cellular longevity with mushroom extracts. Foods 2024, 13(24), Art. No: 244028. DOI: 10.3390/foods13244028
30. Karaltı, İ.; Eraslan, E.C.; Sarıdoğan, B.G.Ö.; Akata, I.; Sevindik, M. Total antioxidant, antimicrobial, antiproliferative potentials and element contents of wild mushroom Candolleomyces candolleanus (Agaricomycetes) from Turkey. Int. J. Med. Mushrooms 2022, 24(12), 69-76. DOI: 10.1615/IntJMedMushrooms.2022045389
31. Akata, I.; Ergonul, B.; Kalyoncu, F. Chemical compositions and antioxidant activities of 16 wild edible mushroom species grown in Anatolia. Int. J. Pharmacol. 2012, 8(2), 134–138. DOI: 10.3923/ijp.2012.134.138
32. Gürgen, A.; Ünal, O.; Sevindik, M. Biological activities of the golden chantarelle mushroom Cantharellus cibarius (Agaricomycetes) extracts obtained as a result of single and multi-objective optimization studies. Int. J. Med. Mushrooms 2024, 26(12), 63–74. DOI: 10.1615/IntJMedMushrooms.2024055569
33. Sevindik, M.; Gürgen, A.; Khassanov, V.T.; Bal, C. Biological activities of ethanol extracts of Hericium erinaceus obtained as a result of optimization analysis. Foods 2024, 13(10), Art. No: 1560. DOI: 10.3390/foods13101560
34. Sevindik, M.; Bal, C.; Krupodorova, T.; Gürgen, A.; Eraslan, E.C. Extract optimization and biological activities of Otidea onotica using artificial neural network–genetic algorithm and response surface methodology techniques. BMC Biotechnol. 2025, 25, Art. No: 25. DOI: 10.1186/s12896-025-00960-y
35. Ünal, O.; Gürgen, A.; Krupodorova, T.; Sevindik, M.; Kabaktepe, Ş.; Akata, I. Optimization of Phellinus hartigii extracts: Biological activities and phenolic content analysis. BMC Complement. Med. Ther. 2025, 25(1), Art. No: 113. DOI: 10.1186/s12906-025-04851-9
36. Sevindik, M.; Bal, C.; Ünal, O.; Eraslan, E.C. Bioactivity of the Gray Knight Mushroom Tricholoma terreum (Agaricomycetes): Antioxidant, antiproliferative, and enzyme inhibition potential. Int. J. Med. Mushrooms 2025, 27, 75–84. DOI: 10.1615/IntJMedMushrooms.2025058982
37. Gürgen, A.; Sevindik, M. Single and multi-objective optimization of the Red Pine Mushroom Lactarius deliciosus (Agaricomycetes) extraction conditions using artificial intelligence methods and biological activities of optimized extracts. Int. J. Med. Mushrooms 2025, 27(2), 59–73. DOI: 10.1615/IntJMedMushrooms.2024057054
38. Gürgen, A.; Sevindik, M. Application of artificial neural network coupling multiobjective particle swarm optimization algorithm to optimize Pleurotus ostreatus extraction parameters. J. Food Process. Preserv. 2022, 46, Art. No: e16949. DOI: 10.1111/jfpp.16949
39. Rai, S.; Mishra, D.; Singh, P.; Vamanu, E.; Singh, M. Therapeutic applications of mushrooms and their biomolecules along with a glimpse of in silico approach in neurodegenerative diseases. Biomed. Pharmacother. 2021, 137, Art. No: 111377. DOI: 10.1016/j.biopha.2021.111377
40. Çolak, S.; Çömlekçioğlu, N.; Aygan, A.; Kocabaş, Y.Z.; Çömlekçioğlu, U. Identification of phenolic content, fatty-acid compositions, antioxidant and antimicrobial activity, and enzyme inhibition effects of endemic Achillea boissieri and Achillea cucullata. Pharm. Chem. J. 2025, 59, 74-85. DOI: 10.1007/s11094-025-03366-4
41. Ersoy, E.; Boğa, M.; Kaplan, A.; Mataracı Kara, E.; Eroğlu Özkan, E.; Demirci Kayıran, S. LC‐HRMS profiling of phytochemicals with assessment of antioxidant, anticholinesterase, and antimicrobial potentials of Astragalus brachystachys DC. Chem. Biodivers. 2025, 22(2), Art. No: e202401853. DOI: 10.1002/cbdv.202401853
42. Jiang, X.; Li, S.; Feng, X.; Li, L.; Hao, J.; Wang, D.; Wang, Q. Mushroom polysaccharides as potential candidates for alleviating neurodegenerative diseases. Nutrients 2022, 14(22), Art. No: 4833. DOI: 10.3390/nu14224833
43. Bingül, M.; Ercan, S.; Boğa, M.; Arslan, Z.; Tuneğ, M.; Akocak, S.; Şahin, H. The anticholinesterase perspective of dimethoxyindole-based benzenesulfonamides: Synthesis, biological investigation and molecular docking applications. Chem. Select 2024, 9, Art. No: e202400895. DOI: 10.1002/slct.202400895
44. Mushtaq, W.; Baba, H.; Akata, İ.; Sevindik, M. Antioxidant potential and element contents of wild edible mushroom Suillus granulatus. KSÜ Tarım Doğa Derg. 2020, 23(3), 592–595. DOI: 10.18016/ksutarimdoga.vi.653241
45. Wasser, S.P.; Sokolov, D.; Reshetnikov, S.V.; Timor-Tismenetsky, M. Dietary supplements from medicinal mushrooms: Diversity of types and variety of regulations. Int. J. Med. Mushrooms 2000, 2(1). DOI: 10.1615/IntJMedMushr.v2.i1.10
46. Al-Obaidi, J.; Jambari, N.; Ahmad-Kamil, E. Mycopharmaceuticals and nutraceuticals: Promising agents to improve human well-being and life quality. J. Fungi 2021, 7(7), Art. No: 503. DOI: 10.3390/jof7070503
47. Šíma, J.; Vondruška, J.; Svoboda, L.; Šeda, M.; Rokos, L. The accumulation of risk and essential elements in edible mushrooms Chlorophyllum rhacodes, Suillus grevillei, Imleria badia, and Xerocomellus chrysenteron growing in the Czech Republic. Chem. Biodivers. 2019, 16(2), Art. No: e1800478. DOI: 10.1002/cbdv.201800478
48. Satarug, S.; Gobe, G.C.; Vesey, D.A.; Phelps, K.R. Cadmium and lead exposure, nephrotoxicity, and mortality. Toxics 2020, 8(4), Art. No: 86. DOI: 10.3390/toxics8040086
49. Bal, C.; Eraslan, E.C.; Sevindik, M. Antioxidant, antimicrobial activities, total phenolic and element contents of wild edible mushroom Bovista nigrescens. Prospects Pharm. Sci. 2023, 21(2), 37–41. DOI: 10.56782/pps.139
50. Korkmaz, A.İ.; Bal, C.; Krupodorova, T.; Yüzbaşıoğlu, M.A.; Sarıdoğan, B.G.Ö.; Sevindik, M. Some biological activities and element levels of Lycoperdon pratense. Prospects Pharm. Sci. 2024, 22(2), 39–44. DOI: 10.56782/pps.189
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