Evaluation of Antioxidant Potential, Anticholinesterase Activity and Element Content of Chlorophyllum rhacodes Species

Ilgaz Akata; Hayri Baba; Şanlı Kabaktepe; Mustafa Sevindik

doi:10.56782/pps.521

Published : 2025-10-05

No. 2025 (Early Access)

Evaluation of Antioxidant Potential, Anticholinesterase Activity and Element Content of Chlorophyllum rhacodes Species

Ilgaz Akata

Hayri Baba

Şanlı Kabaktepe

Mustafa Sevindik

DOI: https://doi.org/10.56782/pps.521

Abstract

In this study, antioxidant potential, anticholinesterase activity and elemental contents of Chlorophyllum rhacodes were evaluated. In order to determine the antioxidant capacity, total antioxidant level (TAS), total oxidant level (TOS), oxidative stress index (OSI), DPPH radical scavenging activity and FRAP ferric reducing power analyses were applied. According to the obtained results, TAS value was determined as 3.125±0.060 mmol/L, TOS value as 6.821±0.062 µmol/L, OSI value as 0.218±0.003, DPPH activity as 55.430±1.438 mg TE/g and FRAP value as 72.540±1.461 mg TE/g. These data revealed that C. rhacodes showed a significant level of antioxidant activity. In the evaluation of anticholinesterase activity, inhibitory effects against AChE and BChE enzymes were determined and IC₅₀ values were determined as 94.330±1.536 µg/mL and 128.397±1.556 µg/mL, respectively. These results show that C. rhacodes shows limited inhibitory effect on these enzymes but may have a certain level of anticholinesterase potential. According to the results of elemental analysis, Cd (14.78±1.53), Cr (33.17±1.42), Mn (7.35±0.52), Cu (155.77±2.34), Fe (469.30±15.75), Pb (6.98±0.54), Ni (3.66±0.31) and Zn (76.18±2.25) mg/kg levels were detected in mushroom samples. In particular, it was observed that Cd and Cu levels were above the upper limits specified in the literature. This situation shows that C. rhacodes tends to accumulate some heavy metals and may be highly sensitive to environmental pollution. In conclusion, C. rhacodes species exhibits a biologically remarkable profile with certain antioxidant and anticholinesterase activities and can also be considered as a potential indicator species in the biological monitoring of environmental metal exposure.

Keywords:

Chlorophyllum rhacodes, antioxidant activity, anticholinesterase, Heavy metals, elemental analysis, functional mushroom

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Akata, I., Baba, H., Kabaktepe, Şanlı, & Sevindik, M. (2025). Evaluation of Antioxidant Potential, Anticholinesterase Activity and Element Content of Chlorophyllum rhacodes Species. Prospects in Pharmaceutical Sciences, (2025 (Early Access). https://doi.org/10.56782/pps.521

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Ilgaz Akata

akatailgaz@gmail.com

Affiliation:

Department of Biology, Faculty of Sciences, Ankara University, 06-000 Ankara, Türkiye Turkey

Hayri Baba

hayribaba_68@hotmail.com

Affiliation:

Department of Biology, Faculty of Science and Literature, Hatay Mustafa Kemal University, 31-000 Hatay, Türkiye. Turkey

Şanlı Kabaktepe

sanli.kabaktepe@ozal.edu.tr

Affiliation:

Battalgazi Vocational School of Higher Education, Malatya Turgut Ozal University, 44-000 Malatya, Türkiye. Turkey

Mustafa Sevindik

sevindik27@gmail.com

Affiliation:

a:1:{s:5:"en_US";s:115:"Osmaniye Korkut Ata University, Faculty of Science and Literature, Department of Biology, Osmaniye, 80000, Türkiye";} Turkey

Evaluation of Antioxidant Potential, Anticholinesterase Activity and Element Content of Chlorophyllum rhacodes Species

Ilgaz Akata

Hayri Baba

Şanlı Kabaktepe

Mustafa Sevindik

Abstract

Keywords:

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