Herbal power: antimicrobial, antioxidant and anticholinesterase properties  of Citrullus colocynthis

Emre Sevindik; Orhan Unal; Imran Uysal; Mansur Seymen Seğmenoğlu; Mustafa Sevindik

doi:10.56782/pps.382

Published : 2025-05-14

Vol. 23 No. 2 (2025)

Herbal power: antimicrobial, antioxidant and anticholinesterase properties of Citrullus colocynthis

Emre Sevindik

Orhan Unal

Imran Uysal

Mansur Seymen Seğmenoğlu

Mustafa Sevindik

DOI: https://doi.org/10.56782/pps.382

Abstract

Citrullus colocynthis (L.) Schrad. is a biologically rich plant widely used in traditional medicine. This study aimed to investigate the antimicrobial, antioxidant and anticholinesterase potential and phenolic/flavonoid contents of C. colocynthis. Antimicrobial tests using ethanol and methanol extracts revealed that it exhibited strong antimicrobial effects especially against pathogens such as Staphylococcus aureus, Candida albicans, C. krusei and Acinetobacter baumannii. In addition, antioxidant activity tests showed that ethanol extract had high antioxidant capacity, while methanol extract was moderately effective. Phenolic and flavonoid content analyses showed that ethanol extract contained higher phenolic and flavonoid components. In addition, anticholinesterase activity tests showed that C. colocynthis extract exhibited a significant anticholinesterase effect, which could potentially be useful in the treatment of neurological diseases. These findings indicate that C. colocynthis is a potent natural resource with potential therapeutic benefits and its antimicrobial, antioxidant and anticholinesterase properties are of considerable importance in biomedical applications.

Keywords:

Antialzeihmer, Antimicrobial, Antioxidant, Citrullus colocynthis, Medicinal plants

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Sevindik, E., Unal, O., Uysal, I., Seğmenoğlu, M. S., & Sevindik, M. (2025). Herbal power: antimicrobial, antioxidant and anticholinesterase properties of Citrullus colocynthis. Prospects in Pharmaceutical Sciences, 23(2), 67–73. https://doi.org/10.56782/pps.382

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References

1. Karaman, S.; Ilcim, A.; Comlekcioglu, N. Composition of the essential oils of Salvia aramiensis Rech. fil. and Salvia cyanescens Boiss. & Bal. Pak. J. Bot. 2007, 39(1), Art. No: 169.

2. Vaou, N.; Stavropoulou, E.; Voidarou, C.; Tsigalou, C.; Bezirtzoglou, E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms 2021, 9(10), Art. No: 2041. DOI: 10.3390/microorganisms9102041

3. Kalkan, M.; Aygan, A.; Çömlekçioglu, N.; Çömlekçioğlu, U. Olea europaea Yapraklarının Bazı Biyoaktif Özelliklerinin Araştırılması, Antimikrobiyal ve Enzim İnhibisyon Etkinliğinin İncelenmesi. Turkish Journal of Agriculture-Food Science and Technology 2023, 11(3), 496-504. DOI: 10.24925/turjaf.v11i3.496-504.5828

4. Nwozo, O.S.; Effiong, E.M.; Aja, P.M.; Awuchi, C.G. Antioxidant, phytochemical, and therapeutic properties of medicinal plants: A review. Int. J. Food Prop. 2023, 26(1), 359-388. DOI: 10.1080/10942912.2022.2157425

5. Mohammed, F.S.; Pehlivan, M.; Sevindik, E.; Akgul, H.; Sevindik, M.; Bozgeyik, I.; Yumrutas, O. Pharmacological properties of edible Asparagus acutifolius and Asparagus officinalis collected from North Iraq and Turkey (Hatay). Acta Aliment. 2021, 50(1), 136-143. DOI: 10.1556/066.2020.00204

6. Doğan, M.; Mohammed, F.S.; Uysal, İ.; Mencik, K.; Kına, E.; Pehlivan, M.; Sevindik, M. Total antioxidant status, antimicrobial and antiproliferative potentials of Viola odorata (Fragrant Violet). J. Fac. Pharm. Ankara 2023, 47(3), 784-791. DOI: 10.33483/jfpau.1161440

7. Sevindik, M.; Mohammed, F.S.; Uysal, I. Autism: plants with neuro-psychopharmacotherapeutic potential. Prospect. Pharm. Sci. 2023, 21(3), 38-48. DOI: 10.56782/pps.143

8. El-Chaghaby, G.A.; Mohammed, F.S.; Rashad, S.; Uysal, I.; Koçer, O.; Lekesiz, Ö.; Sevindik, M. Genus Hypericum: General Properties, Chemical Contents and Biological Activities. Egypt. J. Botany 2024, 64(1), 1-26. DOI: 10.21608/ejbo.2023.217116.2378

9. Colak, S.; Comlekcioglu, N.; Aygan, A.; Kocabas, Y.Z.; Comlekcioglu, U. Phytochemical Properties and Bioactive Potential of Various Astragalus spp. from Turkey. Food Biosci. 2025, 64, Art. No: 105901. DOI: 10.1016/j.fbio.2025.105901

10. Mandour, Y.M.; Refaat, E.; Hassanein, H.D.; Anticancer activity, phytochemical investigation and molecular docking insights of Citrullus colocynthis (L.) fruits. Sci. Rep. 2023, 13, Art. No: 20038. DOI: 10.1038/s41598-023-46867-6

11. Cheng, X.; Qin, M.; Chen, R.; Jia, Y.; Zhu, Q.; Chen, G.; Wang, A.; Ling, B.; Rong, W. Citrullus colocynthis (L.) Schrad.: A Promising Pharmaceutical Resource for Multiple Diseases. Molecules 2023, 28, 6221. DOI: 10.3390/molecules28176221

12. Asyaz, S.; Khan, F.U.; Hussain, I.; Khan, M.A.; Khan, I.U. Evaluation of chemical analysis profile of Citrullus colocynthis growing in Southern area of Khyber Pukhtunkhwa, Pakistan. World Appl. Sci. J. 2010, 10, 402-405.

13. Sevindik, M.; Uysal, İ.; Sevindik, E.; Krupodorova, T.; Koçer, O.; Ünal, O. Citrullus colocynthis (Bitter-apple): a comprehensive review on general properties, biological activities, phenolic and chemical contents. Not. Sci. Biol. 2024, 16(4), 12202-12202. DOI: 10.55779/nsb16412202

14. Uma, C.; Sekar, K.G. (2014). Phytochemical analysis of a folklore medicinal plant Citrullus colocynthis L (bitter apple). J. Pharmacogn. Phytochem. 2014, 2(6), 195-202.

15. Bauer, A.W.; Kirby, W.M.; Sherris, J.C.; Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966, 45, 493-496. DOI: 10.1093/ajcp/45.4_ts.493

16. Hindler, J.; Hochstein, L.; Howell, A. Preparation of routine media and reagents used in antimicrobial susceptibility testing. Part 1. McFarland standards, p. 5.19.1-5.19.6. In H. D. Isenberg (ed) Clinical microbiology procedures handbook 1992, vol. 1. American Society for Microbiology, Washington, D.C.

17. Matuschek, E.; Brown, D.F.; Kahlmeter, G. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin. Microbiol. Infect. 2014, 20, 255-266. DOI: 10.1111/1469-0691.12373

18. Baba, H.; Sevindik, M.; Dogan, M.; Akgul, H. Antioxidant, antimicrobial activities and heavy metal contents of some Myxomycetes. Fresenius Environ. Bull. 2020, 29(09), 7840-7846.

19. Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 2004, 37(4), 277-285. DOI: 10.1016/j.clinbiochem.2003.11.015

20. Erel, O. A new automated colorimetric method for measuring total oxidant status. Clin. Biochem. 2005, 38(12), 1103-1111. DOI: 10.1016/j.clinbiochem.2005.08.008

21. Sevindik, M. The novel biological tests on various extracts of Cerioporus varius. Fresenius Environ. Bull. 2019, 28(5), 3713-3717.

22. Ellman, G.L.; Courtney, K.D.; Andres Jr, V.; Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961, 7(2), 88-95. DOI: 10.1016/0006-2952(61)90145-9

23. Korkmaz, N.; Koçer, O.; Fathi, S.; Uysal, I.; Sevindik, M. Branched horsetail (Equisetum ramosissimum): some biological activities and total phenolic and flavonoid contents. Prospect. Pharm. Sci. 2024, 22(3), 69-75. DOI: 10.56782/pps.209

24. Sevindik, M.; Bal, C.; Eraslan, E.C.; Uysal, I.; Mohammed, F.S. Medicinal mushrooms: a comprehensive study on their antiviral potential. Prospect. Pharm. Sci 2023, 21(2), 42-56. DOI: 10.56782/pps.141

25. Mohammed, F.S.; Uysal, I.; Sevindik, M. A review on antiviral plants effective against different virus types. Prospect. Pharm. Sci. 2023, 21(2), 1-21. DOI: 10.56782/pps.128

26. Sevindik, M.; Khassanov, V.T.; Sevindik, E.; Uysal, I.; Mohammed, F.S.. Cornelian Cherry (Cornus mas L.): A Comprehensive Review on its Usage Areas, Biological Activities, Mineral, Phenolic and Chemical Contents and Applications. Appl. Fruit Sci. 2024, 66(5), 2061-2071. DOI: 10.1007/s10341-024-01151-3

27. Marzouk, B.; Marzouk, Z.; Mastouri, M.; Fenina, N.; Aouni, M. Comparative evaluation of the antimicrobial activity of Citrullus colocynthis immature fruit and seed organic extracts. Afr. J. Biotechnol. 2011, 10(11), 2130-2134. DOI: 10.5897/AJB10.1412

28. Gurudeeban, S.; Rajamanickam, E.; Ramanathan, T.; Satyavani, K. Antimicrobial activity of Citrullus colocynthis in Gulf of Mannar. Int. J. Curr. Res. 2010, 2(1), 78-81.

29. Tiku, A.R. Antimicrobial compounds and their role in plant defense. In: Molecular aspects of plant-pathogen interaction, Eds: Singh, A.; K. Singh, I.K. 2018, 283-307. DOI: 10.1007/978-981-10-7371-7_13

30. Huang, W.; Wang, Y.; Tian, W.; Cui, X.; Tu, P.; Li, J.; Liu, X. Biosynthesis investigations of terpenoid, alkaloid, and flavonoid antimicrobial agents derived from medicinal plants. Antibiotics 2022, 11(10), Art. No: 1380. DOI: 10.3390/antibiotics11101380

31. Gürgen, A.; Sevindik, M. Single and Multi-Objective Optimization of the Red Pine Mushroom Lactarius deliciosus (Agaricomycetes) Extraction Conditions Using Artificial Intelligence Methods and Biological Activities of Optimized Extracts. Int. J. Med. Mushrooms 2025, 27 (2), 59-73. DOI: 10.1615/IntJMedMushrooms.2024057054

32. Gürgen, A.; Unal, O.; Sevindik, M. Biological activities of the golden chantarelle mushroom Cantharellus cibarius (Agaricomycetes) extracts obtained as a result of single and multi-objective optimization studies. Int. J. Med. Mushrooms 2024, 26(12), 63-74. DOI: 10.1615/IntJMedMushrooms.2024055569

33. Abeyrathne, E.D.N.S.; Nam, K.; Huang, X.; Ahn, D.U. Plant-and animal-based antioxidants’ structure, efficacy, mechanisms, and applications: A review. Antioxidants 2022, 11(5), Art. No: 1025. DOI: 10.3390/antiox11051025

34. Comlekcioglu, N.; Dağlı, F.; Çömlekcioğlu, U.; Aygan, A. Cornus mas ve Rosa canina Meyvelerinin Antioksidan Kapasitesi ve Bazı Fitokimyasal Özellikleri. Turkish Journal of Agriculture-Food Science and Technology 2022, 10(9), 1724-1731. DOI: 10.24925/turjaf.v10i9.1724-1731.5434

35. Dumanović, J.; Nepovimova, E.; Natić, M.; Kuča, K.; Jaćević, V. The significance of reactive oxygen species and antioxidant defense system in plants: A concise overview. Front. Plant Sci. 2021, 11, Art. No: 552969. DOI: 10.3389/fpls.2020.552969

36. Mohammed, F.S.; Sevindik, M.; Uysal, İ.; Sabik, A.E. Quercetin: derivatives, biosynthesis, biological activity, pharmacological and therapeutic effects. Prospect. Pharm. Sci 2023, 21(3), 49-56. DOI: 10.56782/pps.147

37. Kumar, S.; Kumar, D.; Saroha, K.; Singh, N.; Vashishta, B. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharm. 2008, 58(2), 215-220. DOI: 10.2478/v10007-008-0008-1

38. Hussain, A.I.; Rathore, H.A.; Sattar, M.Z.; Chatha, S.A.; ud din Ahmad, F.; Ahmad, A.; Johns, E.J. Phenolic profile and antioxidant activity of various extracts from Citrullus colocynthis (L.) from the Pakistani flora. Ind. Crops Prod. 2013, 45, 416-422. DOI: 10.1016/j.indcrop.2013.01.002

39. Bourhia, M.; Bouothmany, K.; Bakrim, H.; Hadrach, S.; Salamatullah, A.M.; Alzahrani, A.; Benbacer, L. Chemical profiling, antioxidant, antiproliferative, and antibacterial potentials of chemically characterized extract of Citrullus colocynthis L. seeds. Separations 2021, 8(8), Art. No: 114. DOI: 10.3390/separations8080114

40. Mohammed, F.S.; Sevindik, M.; Uysal, I.; Sevindik, E.; Akgül, H. A natural material for suppressing the effects of oxidative stress: biological activities of Alcea kurdica. Biol. Bull. 2022, 49(Suppl 2), S59-S66. DOI: 10.1134/S1062359022140102

41. Gürgen, A.; Sevindik, M.; Krupodorova, T.; Uysal, I.; Unal, O. Biological activities of Hypericum spectabile extract optimized using artificial neural network combined with genetic algorithm application. BMC Biotechnol. 2024, 24(1), Art. No: 83. DOI: 10.1186/s12896-024-00914-w

42. Özcandır, A.; Mohammed, F.S.; Sevindik, M.; Aykurt, C.; Selamoglu, Z.; Akgül, H. (2024). Phenolic composition, total antioxidant, antiradical and antimicrobial potential of endemic Glaucium Alakirensis. Sigma J. Eng. Nat. Sci. 2024, 42(1), 42-48. DOI: 10.14744/sigma.2024.00006

43. Seğmenoğlu, M.S.; Sevindik, M. Antioxidant and antimicrobial potentials of functional food Arum Dioscoridis. Sigma J. Eng. Nat. Sci. 2024, 42(1), 116-120. DOI: 10.14744/sigma.2024.00013

44. Sevindik, M.; Gürgen, A.; Krupodorova, T.; Uysal, İ.; Koçer, O. A hybrid artificial neural network and multi-objective genetic algorithm approach to optimize extraction conditions of Mentha longifolia and biological activities. Sci. Rep. 2024, 14(1), Art. No: 31403. DOI: 10.1038/s41598-024-83029-8

45. Sabik, A.E.; Sevindik, M.; Mohammed, F.S.; Dogan, M. A New Natural Source against A549 Lung Cancer Cells Anthemis cotula and its Biological Activities and Phenolic Contents. Pharm. Chem. J. 2024, 58(7), 1134-1140. DOI: 10.1007/s11094-024-03251-6

46. Eraslan, E.C.; Altuntas, D.; Baba, H.; Bal, C.; Akgül, H.; Akata, I.; Sevindik, M. Some biological activities and element contents of ethanol extract of wild edible mushroom Morchella esculenta. Sigma J. Eng. Nat. Sci. 2021, 39(1), 24-28.

47. Mohammed, F.S.; Sevindik, M.; Uysal, İ.; Çesko, C.; Koraqi, H. Chemical Composition, Biological Activities, Uses, Nutritional and Mineral Contents of Cumin (Cuminum cyminum). Measurement: Food 2024, 14, Art. No: 100157. DOI: 10.1016/j.meafoo.2024.100157

48. Berwal, M.K.; Ram, C.; Gurjar, P.S.; Gora, J.S.; Kumar, R.; Verma, A.K.; Singh, D.; Basile, B.; Rouphael, Y.; Kumar, P. The bioactive compounds and fatty acid profle of bitter apple seed oil obtained in hot, arid environments. Horticulturae 2022, 8(3), Art. No: 259. DOI: 10.3390/horticulturae8030259

49. Balasundram, N.; Sundram, K.; Samman, S. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem. 2006, 99(1), 191-203. DOI: 10.1016/j.foodchem.2005.07.042

50. Dai, J.; Mumper, R.J. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010, 15(10), 7313-7352. DOI: 10.3390/molecules15107313

51. Chang, C.C.; Yang, M.H.; Wen, H.M.; Chern, J.C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Compos. Anal. 2002, 10(3), 178-182. DOI: 10.38212/2224-6614.2748

52. Bejenaru, L.E.; Biţă, A.; Mogoşanu, G.D.; Segneanu, A.E.; Radu, A.; Ciocîlteu, M.V.; Bejenaru, C. Polyphenols Investigation and Antioxidant and Anticholinesterase Activities of Rosmarinus officinalis L. Species from Southwest Romania Flora. Molecules 2024, 29(18), Art. No: 4438. DOI: 10.3390/molecules29184438

53. Cartuche, L.; Vallejo, C., Castillo, E.; Cumbicus, N.; Morocho, V. Chemical profiling of Drimys granadensis (Winteraceae) essential oil, and their antimicrobial, antioxidant, and anticholinesterase properties. Plants 2024, 13(13), Art. No: 1806. DOI: 10.3390/plants13131806

54. Shadat, A.A.; Ibrahim, A.Y.; Ezzeldin, E.; Alsaid, M.S. Acetylcholinesterase inhibition and antioxidant activity of some medicinal plants for treating neuro degenarative disease. Afr. J. Tradit. Complement. Altern. Med. 2015, 12(3), 97-103. DOI: 10.4314/ajtcam.v12i3.12

55. Orhan, I.; Şener, B.; Choudhary, M.I.; Khalid, A. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J. Ethnopharmacol. 2004, 91(1), 57-60. DOI: 10.1016/j.jep.2003.11.016

56. Mukherjee, P.K.; Kumar, V.; Mal, M.; Houghton, P.J. Acetylcholinesterase inhibitors from plants. Phytomedicine 2007, 14(4), 289-300. DOI: 10.1016/j.phymed.2007.02.002

Emre Sevindik

ph.d-emre@hotmail.com

Bio Statement (e.g., department and rank)

Department of Agricultural Biotechnology, Faculty of Agriculture, Aydın Adnan Menderes University, 09-100 Aydın, Türkiye

Orhan Unal

ounal@akdeniz.edu.tr

Affiliation:

Department of Biology, Faculty of Science, Akdeniz University, 07-070 Antalya, Türkiye Turkey

Imran Uysal

uysal-imran@hotmail.com

Affiliation:

Department of Food Processing, Bahçe Vocational School, Osmaniye Korkut Ata University, 80-500 Osmaniye, Türkiye Turkey

Mansur Seymen Seğmenoğlu

mansurseymen@gmail.com

Affiliation:

Department of Nursing, Faculty of Health Sciences, Osmaniye Korkut Ata University, 80-000 Osmaniye, Turkey Turkey

Mustafa Sevindik

sevindik27@gmail.com

Affiliation:

a:1:{s:5:"en_US";s:115:"Osmaniye Korkut Ata University, Faculty of Science and Literature, Department of Biology, Osmaniye, 80000, Türkiye";} Turkey

Herbal power: antimicrobial, antioxidant and anticholinesterase properties of Citrullus colocynthis

Emre Sevindik

Orhan Unal

Imran Uysal

Mansur Seymen Seğmenoğlu

Mustafa Sevindik

Abstract

Keywords:

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